gidA
168

glucose-inhibited division protein, part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with ThdF), synthesis of mnm5s2U at the Wobble base position (U34) in tRNAs

Locus

BSU_41010

Molecular weight

69.58 kDa

Isoelectric point

6.33

Protein length

628 aaSequenceBlast

Gene length

1887 bpSequenceBlast

Function

tRNA modification

Product

part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with ThdF)

Essential

Synonyms

gidA, mnmG

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0445 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

4,209,603 4,211,489

Phenotypes of a mutant

inactivation of ''gidA'' reduces sporulation efficiency to 23.9% that of wild type cells; longer mother cells, with some aberrant septa and forespores PubMed

The protein

Catalyzed reaction/ biological activity

(s²U34 in tRNA) + GTP + FAD + CH₂-THF + NADH --> 5-carboxymethylaminomethyl-2- thiouridine PubMed

Protein family

MnmG family (with TrmFO, according to UniProt)

Cofactors

FAD (according to UniProt)

Structure

3CES (PDB) (the protein of E. coli, 52.6% identity) PubMed

P25812 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)