thdF
168

GTP-binding protein, part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with GidA), synthesis of mnm⁵s²U at the Wobble base position (U34) in tRNAs

Locus

BSU_41020

Molecular weight

50.81 kDa

Isoelectric point

4.64

Protein length

459 aaSequenceBlast

Gene length

1380 bpSequenceBlast

Function

tRNA modification

Product

part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with GidA)

Essential

Synonyms

thdF, mnmE, trmE

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0486 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

4,211,510 4,212,889

Phenotypes of a mutant

inactivation of ''thdF'' reduces sporulation efficiency to 21.9% that of wild type cells; longer mother cells, some with aberrant septa and forespores PubMed

The protein

Catalyzed reaction/ biological activity

Binds and hydrolyzes GTP and readily exchanges GDP for GTP

(s²U34 in tRNA) + GTP + FAD + CH₂-THF + NADH --> 5-carboxymethylaminomethyl-2- thiouridine PubMed

Protein family

TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily (according to UniProt)

Domains

TrmE-type G domain (aa 221-380) (according to UniProt)

Structure

1XZP (PDB) (from Thermotoga maritima, 44% identity) PubMed

P25811 (AlphaFold)

Paralogous protein(s)

Obg, YqeH, YnbA, EngA, YyaF, Era, YsxC, RbgA

Localization

cytoplasm (according to Swiss-Prot)

Additional information

Information on the corresponding protein from E. coli: EcoCyc

MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

Expression and Regulation

Operons

Genes

thdF-gidA-gidB-noc

Description

PubMed

Regulation

constitutively expressed PubMed

Regulatory mechanism

ComK: activation, PubMed, in comK regulon

DnaA: repression, PubMed, in dnaA regulon

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