thrD
168

aspartokinase III

Locus

BSU_03790

Molecular weight

49.66 kDa

Isoelectric point

4.78

Protein length

454 aaSequenceBlast

Gene length

1365 bpSequenceBlast

Function

biosynthesis of threonine

Product

aspartokinase III

Essential

E.C.

2.7.2.4

Synonyms

thrD, yclM

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0527 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

430,623 431,987

The protein

Catalyzed reaction/ biological activity

ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)

Protein family

aspartokinase family (with DapG and LysC, according to UniProt)

Domains

2 ACT domains (aa 312-388, aa 389-454) (according to UniProt)

Structure

3TVI (PDB) (from Clostridium acetobutylicum, 37% identity) PubMed

P94417 (AlphaFold)

Effectors of protein activity

inhibited by the simultaneous presence of threonine and lysine PubMed

Expression and Regulation

Operons

Genes

thrD

Description

PubMed

Regulation

expressed in the presence of lysine or cysteine (ThrR) PubMed

Regulatory mechanism

CodY: repression, PubMed, in codY regulon

ThrR: repression, PubMed, in thrR regulon

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Biological materials

Mutant

MGNA-C009 (yclM::erm), available at the NBRP B. subtilis, Japan

BKE03790 (ΔthrD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTACATCTCCTAATG, downstream forward: _UP4_TAATCGTACATAAATAGCGG

BKK03790 (ΔthrD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTACATCTCCTAATG, downstream forward: _UP4_TAATCGTACATAAATAGCGG

LacZ fusion

pBP318 (in pAC5), available in Fabian Commichau's lab PubMed

References

Reviews

Original Publications

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Time of last update: 2025-04-06 14:06:13

Author of last update: Jstuelk

thrD 168

thrD
168