cwlO

cwlO
168

D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation

Locus
BSU_34800
Molecular weight
50.87 kDa
Isoelectric point
5.33
Protein length
473 aaSequenceBlast
Gene length
1422 bpSequenceBlast
Function
cell wall synthesis, cell elongation
Product
endopeptidase-type autolysin
Essential
no
Synonyms
cwlO, yzkA, yvcE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3883 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,574,363  3,575,784
Phenotypes of a mutant
a cwlO lytE mutant is not viable PubMed
a cwlO sigI mutant is not viable, due to lack of expression of lytE PubMed
a cwlO rasP mutant is not viable, due to lack of processing of RsgI and the resulting inactivity of SigI PubMed
a cwlO ecsA mutant is not viable, due to lack of processing of RsgI and the resulting inactivity of SigI PubMed
a cwlO ecsB mutant is not viable, due to lack of processing of RsgI and the resulting inactivity of SigI PubMed
shorter, fatter cells, this can be rescued by addition of Mg(2+) PubMed
the mutant cells are thicker and shorter than wild type cells PubMed
loss of genetic competence PubMed
defective in Swarming motility PubMed
improved survival after DNA replication arrest imposed by inhibition of PolC activity PubMed
poor growth of a ltaS cwlO double mutant PubMed
The protein
Catalyzed reaction/ biological activity
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) PubMed
degradation of gamma-polyglutamic acid PubMed
Protein family
Peptidase C40 family (according to UniProt)
Domains
C-terminal D,L-endopeptidase domain (NlpC/P60 domain) PubMed
Structure
P40767 (AlphaFold)
Effectors of protein activity
CwlO requires activation by FtsE-FtsX PubMed
activity requires functional Mbl PubMed
both enzymatic activities are inhibited by interaction with IseA PubMed
Localization
extracellular (signal peptide) PubMed
localizes to the outer lateral sidewall of the cell (via the N-terminal domain) PubMed
cell membrane in a FtsX-dependent manner PubMed
Expression and Regulation
Operons
Genes
cwlO
Description
PubMed
Regulation
expressed during exponential growth (WalR) PubMed
the leader mRNA is processed by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
WalR: activation, PubMed, in walR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

cwlO

2025-03-17 13:52:15

Jstuelk

119

7e09d7d3f00ab2b73e135067be0bda5f80d9f891

3DC8D2D0BCFFCE0B13AA82019E60215F6FEDB87D

Biological materials
Mutant
GP2642 (cwlO::aphA3), available in Jörg Stülke's lab
MGNA-B643 (yvcE::erm), available at the NBRP B. subtilis, Japan
BKE34800 (cwlO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTTATATCCTCCCTT,  downstream forward: _UP4_TAATAAATATGACAAGGGCC
BKK34800 (cwlO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTTATATCCTCCCTT,  downstream forward: _UP4_TAATAAATATGACAAGGGCC
References
Reviews
Loading
Original Publications
Loading

4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872

Page visits: 9732

Time of last update: 2025-04-06 00:35:08

Author of last update: Jstuelk