ezrA

ezrA
168

negative regulator of FtsZ ring formation

Locus
BSU_29610
Molecular weight
64.82 kDa
Isoelectric point
4.76
Protein length
562 aaSequenceBlast
Gene length
1689 bpSequenceBlast
Function
control of FtsZ ring formation
Product
FtsZ-interacting protein, member of the divisome
Essential
no
Synonyms
ezrA, ytwP
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4477 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,029,729 → 3,031,417
Phenotypes of a mutant
altered localization of FtsZ and PBP1 PubMed
growth defects, elongated cells PubMed
the ezrA mutation is synthetically lethal with a sepF mutation PubMed
a whiA ezrA double mutant grows poorly, and the cells are filamentous PubMed
zapA and ezrA mutations are synthetically lethal, this can be suppressed by a FtsZ-K86E mutant protein PubMed
depletion of ''pdhA and deletion of ezrA'' have a strong synthetic defect in cell division PubMed
an ezrA yrrS double mutant has a severe growth defect PubMed
an ezrA facZ double mutant has a severe growth defect PubMed
an ezrA yerH double mutant has a severe growth defect PubMed
The protein
Catalyzed reaction/ biological activity
inhibits FtsZ polymerization PubMed
recruits PBP1]] to the division septum PubMed
activates PrkC PubMed
Protein family
EzrA family (single member, according to UniProt)
Structure
4UXV (PDB) (the 60 kDa cytoplasmic domain) PubMed
O34894 (AlphaFold)
Effectors of protein activity
degradation of EzrA involves FtsH PubMed
Localization
cell membrane (integral) PubMed
1 transmembrane domain at the N-terminus PubMed
Expression and Regulation
Operons
Genes
ezrA
Description
PubMed
Regulation
constitutively expressed PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
degradation of EzrA involves FtsH PubMed
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ezrA

2025-04-22 22:27:35

ghost

104

f3c1e0849cc17835d5e01c5c0e5588c9e673eaf4

9DFFF931CD66242A6C13173D3BB37BB5FBCF0488

Biological materials
Mutant
MGNA-A428 (ytwP::erm), available at the NBRP B. subtilis, Japan
1A805 ( ezrA::kan), PubMed, available at BGSC
1A1279 (ezrA::spec), PubMed, available at BGSC
1A1280 (ezrA-gfp), PubMed, available at BGSC
BKE29610 (ΔezrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG,  downstream forward: _UP4_TAGATAATCACGACCATGAA
BKK29610 (ΔezrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG,  downstream forward: _UP4_TAGATAATCACGACCATGAA
References
Reviews
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Original Publications
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Page visits: 6811

Time of last update: 2025-04-25 07:21:57

Author of last update: Jstuelk