Difference between revisions of "Amj"
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| − | * '''Description:''' | + | * '''Description:''' lipid II flippase, alternative to [[MurJ]]<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || lipid II flippase |
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || export of lipid II |
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04230 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04230 amj] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 10.596 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 10.596 | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[cell wall synthesis]]}}, | ||
| + | {{SubtiWiki category|[[biosynthesis of cell wall components]]}}, | ||
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}, | {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}, | ||
{{SubtiWiki category|[[membrane proteins]]}} | {{SubtiWiki category|[[membrane proteins]]}} | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * an ''[[amj]] [[murJ]]'' double mutant is not viable {{PubMed|25918422}} | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Operon:''' ''ydaH'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed] | * '''Operon:''' ''ydaH'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed] | ||
| − | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydaH_474731_475540_1 | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydaH_474731_475540_1 amj] {{PubMed|22383849}} |
* '''Sigma factor:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed] | * '''Sigma factor:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed] | ||
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=References= | =References= | ||
| − | <pubmed>17434969,,17434969, </pubmed> | + | <pubmed>17434969,25918422,17434969, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 06:58, 30 April 2015
- Description: lipid II flippase, alternative to MurJ
| Gene name | ydaH |
| Synonyms | |
| Essential | no |
| Product | lipid II flippase |
| Function | export of lipid II |
| Gene expression levels in SubtiExpress: amj | |
| MW, pI | 29 kDa, 10.596 |
| Gene length, protein length | 807 bp, 269 aa |
| Immediate neighbours | bsrC, ydzA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04230
Phenotypes of a mutant
Database entries
- BsubCyc: BSU04230
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU04230
- Structure:
- UniProt: P96581
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon: ydaH PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Alexander J Meeske, Lok-To Sham, Harvey Kimsey, Byoung-Mo Koo, Carol A Gross, Thomas G Bernhardt, David Z Rudner
MurJ and a novel lipid II flippase are required for cell wall biogenesis in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2015, 112(20);6437-42
[PubMed:25918422]
[WorldCat.org]
[DOI]
(I p)
Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969]
[WorldCat.org]
[DOI]
(P p)
