gabR

gabR
168

transcription activator of gabT-gabD, repressor of gabR (MocR/ GabR family)

Locus
BSU_03890
Molecular weight
55.00 kDa
Isoelectric point
6.71
Protein length
479 aaSequenceBlast
Gene length
1440 bpSequenceBlast
Function
regulation of gamma-amino butyric acid utilization
Product
transcription regulator
Essential
no
Synonyms
gabR, ycnF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
440,025 → 441,464
The protein
Catalyzed reaction/ biological activity
transcription activation of the gabT-gabD operon in the presence  of GABA and PLP, transcription repression of gabR PubMed
Protein family
MocR/ GabR family PubMed
class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Domains
N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the GntR family) PubMed
C-terminal domain is homologous to PLP-binding large domain of aminotransferases PubMed
HTH gntR-type domain (aa 14-82) (according to UniProt)
Cofactors
gamma-aminobutyric acid, PLP PubMed
Structure
4MGR (PDB) (the apo-protein) PubMed
4N0B (PDB) (the complex with PLP) PubMed
P94426 (AlphaFold)
Effectors of protein activity
activation of ''gabT-gabD'' expression is triggered by gamma-amino butyrate and pyridoxalphosphate PubMed
in the absence of GABA, GabR represses gabT-gabD expression, whereas binding of GABA to GabR results in a conformational change resulting in activation of transcription PubMed
Expression and Regulation
Operons
Genes
gabR
Description
PubMed
Regulatory mechanism
GabR: negative autoregulation, PubMed, in gabR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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gabR

2025-04-07 06:16:58

Jstuelk

88

c17c8cfd4239e1c6001df55dbca3fda04d57fc4d

506000345DBD8D9CB0D46D6A4930AD58E33B5DFD

Biological materials
Mutant
MGNA-C014 (ycnF::erm), available at the NBRP B. subtilis, Japan
BKE03890 (ΔgabR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGTAGTTTCTCCTTC,  downstream forward: _UP4_AAAATCCCCGTTACAGGGGA
BKK03890 (ΔgabR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGTAGTTTCTCCTTC,  downstream forward: _UP4_AAAATCCCCGTTACAGGGGA
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
References
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C7C36FAC0CE72226960FC7E8E016B8EC77AF1036

Page visits: 7473

Time of last update: 2025-04-07 06:24:07

Author of last update: Jstuelk