tapA

tapA
168

primer for TasA oligomerization

Locus
BSU_24640
Molecular weight
28.92 kDa
Isoelectric point
6.68
Protein length
253 aaSequenceBlast
Gene length
762 bpSequenceBlast
Function
biofilm formation
Product
primer for TasA oligomerization
Essential
no
Synonyms
tapA, yqhD, yqxM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
2,554,486  2,555,247
Phenotypes of a mutant
no biofilm formation PubMed
The protein
Catalyzed reaction/ biological activity
binds TasA to initiate TasA oligomerization PubMed
Structure
6HQC (PDB) (aa 75 ... 190) PubMed
6QAY (PDB) (NMR structure) PubMed
8AIF (PDB) (aa 71 ... 190) PubMed
P40949 (AlphaFold)
Localization
attached to the cell surface (on the outside of the cell), associated with peptidoglycan  PubMed
secretion requires SipW PubMed
Expression and Regulation
Operons
Genes
tapA-sipW-tasA
Description
PubMed
Regulation
repressed by casamino acids PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
SinR: repression, PubMed, in sinR regulon
RemA: activation, PubMed, in remA regulon
LutR: activation, PubMed, in lutR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
expression of the operon is localized to a ring near the periphery of the biofilm PubMed
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tapA→tasA

2025-04-05 17:22:14

Jstuelk

153

5d4b2839ae781570552ba6a28b48e7489d628dd2

25F0FAE210D5101B1935E4625742633C173FC509

Biological materials
Mutant
MGNA-C429 (yqhD::erm), available at the NBRP B. subtilis, Japan
BKE24640 (tapA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTTACCTCCTGTAAA,  downstream forward: _UP4_AGCAATGAAGCTGATCAGTA
BKK24640 (tapA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTTACCTCCTGTAAA,  downstream forward: _UP4_AGCAATGAAGCTGATCAGTA
LacZ fusion
pGP1926 (in pAC6), available in Jörg Stülke's lab
References
Reviews
Arnaouteli S, Bamford NC, Stanley-Wall NR, Kovács ÁTBacillus subtilis biofilm formation and social interactions.Nature reviews. Microbiology. 2021 Apr 6; . PMID: 33824496
Hobley L, Harkins C, MacPhee CE, Stanley-Wall NR Giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes. FEMS microbiology reviews. 2015 Sep; 39(5):649-69. doi:10.1093/femsre/fuv015. PMID:25907113
Driks A Tapping into the biofilm: insights into assembly and disassembly of a novel amyloid fibre in Bacillus subtilis. Molecular microbiology. 2011 Jun; 80(5):1133-6. doi:10.1111/j.1365-2958.2011.07666.x. PMID:21488983
Original Publications
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Time of last update: 2025-04-07 03:27:53

Author of last update: Jstuelk