rnc

rnc
168

RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria

Locus
BSU_15930
Molecular weight
28.28 kDa
Isoelectric point
8.08
Protein length
249 aaSequenceBlast
Gene length
750 bpSequenceBlast
Function
processing and degradation of RNA molecules
Product
endoribonuclease III
Essential
yes
Synonyms
rnc, rncS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0571 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,665,710 → 1,666,459
Phenotypes of a mutant
essential PubMed
the ''rnc'' gene can be deleted in strains cured of the Skin element and SP-beta prophage (or upon deletion of the toxin genes ''txpA and yonT'') PubMed
Additional information
RNAs that are targeted by RNase III PubMed
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
cleaves 16S rRNA 2 nts downstream of the 3' end PubMed
cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''scr'')
degradation of ''txpA'' mRNA-FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0 RNA duplex (encoded on the Skin element) PubMed
degradation of ''yonT mRNA-SR6'' RNA duplex (encoded on the [[SP-beta prophage]]) PubMed
degradation of the ''roxS-ytdI and roxS-sucC'' RNA duplexes PubMed
cleaves RNA within long-range intramolecular structures PubMed
Protein family
ribonuclease III family (single member, according to UniProt)
Domains
RNase III domain (aa 20-149) (according to UniProt)
DRBM domain (aa 175-222) (according to UniProt)
Structure
1O0W (PDB) (from Thermotoga maritima, 41% identity)
P51833 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
rnc-smc-ftsY
Description
PubMed
Regulation
expressed during vegetative growth and during sporulation in the mother cell (SigK, GerE) PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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rnc→ftsY

2025-04-05 15:29:58

ghost

142

6bc011f23705f0484f05e78a9ae631b907169aff

E7BDA851062351C16952CEF8C156BA6388FED18F

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab, PubMed
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage
Ciaran Condon, IBPC, Paris, France Homepage
References
Reviews
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Original Publications
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BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981

Page visits: 5356

Time of last update: 2025-04-10 20:16:06

Author of last update: Jstuelk