dacB

dacB
168

sporulation-specific Class C penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase

Locus
BSU_23190
Molecular weight
42.92 kDa
Isoelectric point
9.73
Protein length
382 aaSequenceBlast
Gene length
1149 bpSequenceBlast
Function
control of peptide cross-linking in spore peptidoglycan
Product
penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase
Essential
no
Synonyms
dacB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1686 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,423,383  2,424,531
Phenotypes of a mutant
inactivation of ''dacB'' reduces sporulation efficiency to 19.3% that of wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
modifies degree of cross-linking of glycan strands in peptidoglycan PubMed
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)
Protein family
Peptidase S11 family (according to UniProt)
Structure
3MFD (PDB) (beta-lactamase superfamily domain)
P35150 (AlphaFold)
Paralogous protein(s)
DacF
Localization
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
dacB-spmA-spmB
Description
PubMed
Regulation
expressed early during sporulation in the mother cell (SigE) PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
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dacB→spmB

2025-03-30 21:40:55

ghost

91

7b91c54260f7f0e53a5c4144c6ab48e405116b3d

C9960B306C3A092D8114C6E83F0241527A83EB89

Biological materials
Mutant
MGNA-A098 (dacB::erm), available at the NBRP B. subtilis, Japan
1A744 ( dacB::cat), PubMed, available at BGSC
1A745 ( dacB::cat), PubMed, available at BGSC
BKE23190 (dacB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG,  downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC
BKK23190 (dacB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG,  downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC
References
Reviews
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Original Publications
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76825D77907E8CE47B17ED56D7E2A348B1ADA5EC

Page visits: 4192

Time of last update: 2025-04-04 12:12:13

Author of last update: Jstuelk