Difference between revisions of "GerT"

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|style="background:#ABCDEF;" align="center"|'''Function''' || germination
 
|style="background:#ABCDEF;" align="center"|'''Function''' || germination
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19490 gerT]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 6.342   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 6.342   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 471 bp, 157 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 471 bp, 157 aa  
 
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yojE]]'', ''[[yojC]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yojE]]'', ''[[yojB]]''
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAE01457&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19490 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19490 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19490 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yozR_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yozR_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gerT_2124021_2124494_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:gerT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19490]]
 
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|}
  
 
__TOC__
 
__TOC__
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/>
  
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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[sporulation proteins]]}},
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{{SubtiWiki category|[[germination]]}},
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{{SubtiWiki category|[[membrane proteins]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[SigK regulon]]}}
  
 
=The gene=
 
=The gene=
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* mutants respond poorly to multiple germinants, impaired in early stage of germination {{PubMed|17720779}}
 
=== Database entries ===
 
=== Database entries ===
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19490&redirect=T BSU19490]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yozR.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yozR.html]
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=== Additional information===
 
=== Additional information===
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''
  
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* '''Localization:''' cell membrane (according to Swiss-Prot)
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* '''[[Localization]]:'''
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** cell membrane (according to Swiss-Prot), first assembled in foci, then spread around the developing spore in a [[CotE]]-dependent manner {{PubMed|17720779}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19490&redirect=T BSU19490]
  
 
* '''Structure:'''
 
* '''Structure:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  
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* '''Operon:''' ''gerT'' {{PubMed|22383849}}
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gerT_2124021_2124494_1 gerT] {{PubMed|22383849}}
  
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* '''[[Sigma factor]]:'''  
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* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|17720779}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed|17720779}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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** [[GerE]]: transcription repression {{PubMed|17720779}}
  
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* '''Additional information:'''  
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* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Latest revision as of 13:59, 6 April 2015

  • Description: spore coat protein, involved in germination

Gene name gerT
Synonyms yozR
Essential no
Product spore coat protein
Function germination
Gene expression levels in SubtiExpress: gerT
MW, pI 18 kDa, 6.342
Gene length, protein length 471 bp, 157 aa
Immediate neighbours yojE, yojB
Sequences Protein DNA DNA_with_flanks
Genetic context
YozR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GerT expression.png















Categories containing this gene/protein

sporulation proteins, germination, membrane proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU19490

Phenotypes of a mutant

  • mutants respond poorly to multiple germinants, impaired in early stage of germination PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: eamA transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cell membrane (according to Swiss-Prot), first assembled in foci, then spread around the developing spore in a CotE-dependent manner PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK, GerE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Caitlin C Ferguson, Amy H Camp, Richard Losick
gerT, a newly discovered germination gene under the control of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(21);7681-9
[PubMed:17720779] [WorldCat.org] [DOI] (P p)

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