cotH

cotH
168

protein kinase, required for the assembly of several outer coat proteins

Locus
BSU_36060
Molecular weight
42.65 kDa
Isoelectric point
5.95
Protein length
362 aaSequenceBlast
Gene length
1089 bpSequenceBlast
Function
protection of CotU and CotC in the mother cell
Product
protein kinase
Essential
no
Synonyms
cotH, ywrH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5337 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,716,009 → 3,717,097
Phenotypes of a mutant
overexpression of cotH suppresses the phenotypes of a cotE mutant PubMed
reduced germination efficiency PubMed
The protein
Catalyzed reaction/ biological activity
autophosphorylation with ATP and subsequent phosphorylation of CotB and CotG on serine residues PubMed
Protein family
cotH family (with YisJ, according to UniProt)
Cofactors
Mn2+ PubMed
Structure
5JD9 (PDB) PubMed
Q45535 (AlphaFold)
Localization
inner spore coat
Expression and Regulation
Operons
Genes
cotH-cotB-ywrJ
Description
PubMed
Regulation
expressed late during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed
Regulatory mechanism
GerR: activation, PubMed, in gerR regulon
GerE: repression, PubMed, in gerE regulon
SpoIIID: activation, PubMed, in spoIIID regulon
Sigma factors
SigK: sigma factor, PubMed, in sigK regulon
SigE: sigma factor, PubMed, in sigE regulon
Open in new tab

cotH→ywrJ

2025-04-13 18:49:33

ghost

105

522d2dc3540a057ccb4247a51bdcef8f2aab470a

03DF2C1A39CB01A4DB9919D07A586C7F244BEAFF

Biological materials
Mutant
BKE36060 (ΔcotH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCATAATCCTCCTTACA,  downstream forward: _UP4_TGAATGCGTGAAAATGGGTA
BKK36060 (ΔcotH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCATAATCCTCCTTACA,  downstream forward: _UP4_TGAATGCGTGAAAATGGGTA
References
Reviews
Loading
Original Publications
Loading

D53067098F693669F61E5CCF6C06664580C40C79

Page visits: 4791

Time of last update: 2025-04-14 09:22:56

Author of last update: Jstuelk