tmk

tmk
168

thymidylate kinase

Locus
BSU_00280
Molecular weight
23.73 kDa
Isoelectric point
4.87
Protein length
212 aaSequenceBlast
Gene length
639 bpSequenceBlast
Function
TTP biosynthesis
Product
thymidylate kinase
Essential
yes
E.C.
2.7.4.9
Synonyms
tmk, yaaP
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0125 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
39,159  39,797
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
ATP +  dTMP --> ADP +  dTDP (according to UniProt)
Protein family
Thymidylate kinase family (single member, according to UniProt)
Structure
2CCK (PDB) (from Staphylococcus aureus, 53% identity, 74% similarity) PubMed
P37537 (AlphaFold)
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC
Description
PubMed
Open in new tab

xpaC→yabC

2025-03-25 19:55:04

Jstuelk

196

adcd0181c3bf7f5a66d1644d176d0c60f2121a3a

87CEB0D67D1455F48A42F665A0B9A91B779AEC5D

Genes
tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC
Description
PubMed
Open in new tab

tmk→yabC

2025-04-05 08:43:18

Jstuelk

183

cae86a5ed186d19f5321f28f72d35514a5988afb

E9A27179465E2479F141CE804267881CAB860CC2

Biological materials
Expression vectors
pGP1689: expression of Strep-tmk in E. coli, in pGP172, available in Jörg Stülke's lab
pGP2671: expression of tmk-Strep, in pGP380 ,in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP2401 tmk-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
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5B9984BBCEC1244628DC1467BE26976AD8E06286

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Time of last update: 2025-04-10 18:27:51

Author of last update: Jstuelk