tnrA

tnrA
168

transcriptional pleiotropic regulator (MerR family) involved in global nitrogen regulation

Locus
BSU_13310
Molecular weight
12.99 kDa
Isoelectric point
10.23
Protein length
110 aaSequenceBlast
Gene length
333 bpSequenceBlast
Function
regulation of nitrogen assimilation
Product
transcription activator/ repressor (MerR family)
Essential
no
Synonyms
tnrA, scgR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,397,411  1,397,743
The protein
Protein family
MerR family
Domains
HTH merR-type domain (aa 13-81) (according to UniProt)
Structure
4R22 (PDB) (the TnrA-DNA complex) PubMed
4S0R (PDB) (the GlnA-TnrA complex) PubMed
Q45666 (AlphaFold)
Effectors of protein activity
feedback-inhibited GlnA prevents TnrA from DNA binding
Localization
membrane-associated via NrgA-NrgB under conditions of poor nitrogen supply PubMed
Expression and Regulation
Operons
Genes
tnrA
Description
PubMed
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
Regulatory mechanism
TnrA: activation, PubMed, in tnrA regulon
GlnR: repression, in glnR regulon
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tnrA

2025-04-04 08:07:19

Jstuelk

82

1fb2ce88d9aeaae5edf8bdef96072b105a1a59ed

B48C1C7315DDF40852E7BFA2FD194A721B258FA1

Biological materials
Mutant
GP252 (in frame deletion), available in Jörg Stülke's lab
BKE13310 (tnrA::ermC) (available at the BGSC and in Jörg Stülke's lab) PubMed
GP2262 (tnrA::ermC) (available in Jörg Stülke's lab)
1A851 ( tnrA::erm), PubMed, available at BGSC
1A851 ( tnrA::erm), PubMed, available at BGSC
BKE13310 (tnrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCACCCCTGGATG,  downstream forward: _UP4_TAAATAATAAAAGTCCGGCT
BKK13310 (tnrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCACCCCTGGATG,  downstream forward: _UP4_TAAATAATAAAAGTCCGGCT
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP171 available in Jörg Stülke's lab
pGP229 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab
pGP3818 (expression of TnrA in pBQ200), available in Jörg Stülke's lab
Antibody
available in the Karl Forchhammer lab
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Maria Schumacher, Durham, NC, USA homepage
References
Reviews
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Other original publications
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The TnrA regulon
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857EF1AC54DEA0690C01B7F8CEFFD16312C58F20

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Time of last update: 2025-04-03 03:55:29

Author of last update: MBenda