proH

proH
168

pyrroline-5-carboxylate reductase

Locus
BSU_18480
Molecular weight
31.88 kDa
Isoelectric point
7.75
Protein length
297 aaSequenceBlast
Gene length
894 bpSequenceBlast
Function
osmoadaptive de novo production of proline
Product
pyrroline-5-carboxylate reductase
Essential
no
E.C.
1.5.1.2
Synonyms
proH, yoxE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0345 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,016,845  2,017,738
The protein
Catalyzed reaction/ biological activity
1-pyrroline-5-carboxylate + NAD(P)H + H+ --> L-proline + NAD(P)+ PubMed
Protein family
pyrroline-5-carboxylate reductase family PubMed
Cofactors
NADPH PubMed
Structure
5BSE (PDB) (from Medicago truncatula, 36% identity) PubMed
P0CI77 (AlphaFold)
Effectors of protein activity
feedback-inhibited by proline PubMed
Paralogous protein(s)
ProI
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
proH-proJ
Description
PubMed
Regulation
expressed under conditions of osmotic stress PubMed
induced upon antibiotic stress PubMed
Expression
expression is heterogeneously activated PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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proH→proJ

2025-04-03 09:11:28

Jstuelk

86

7D11E4548AED5470042D4AD4B1A81BAF2078CCD0

AED34EA4995FE0B1F5AA7A879B3AA26AFD68CDB8

Biological materials
Mutant
HB24914 (proH::erm), available in John Helmann's and Jörg Stülke's labs
MGNA-B083 (proH::erm), available at the NBRP B. subtilis, Japan
BKE18480 (proH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCACACGTTTAAAAT,  downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
BKK18480 (proH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCACACGTTTAAAAT,  downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
GP3709 (proH::aphA3 trpC2) available in the Stülke lab
Expression vectors
pGP3800: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
pGP3862: expression of Strep-proH by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
References
Reviews
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Original Publications
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542871C77BE841AA59A65FFE8A44AB85BF9180F0

Page visits: 9860

Time of last update: 2025-04-16 14:20:48

Author of last update: Robert.warneke