uvrA

uvrA
168

excinuclease ABC (subunit A), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations

Locus
BSU_35160
Molecular weight
105.83 kDa
Isoelectric point
5.84
Protein length
957 aaSequenceBlast
Gene length
2874 bpSequenceBlast
Function
DNA repair after UV damage
Product
excinuclease ABC (subunit A)
Essential
no
Synonyms
uvrA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0178 (Galperin et al., 2021)

This gene is a member of the following regulons

LexA regulon

Gene
Coordinates
3,610,064  3,612,937
Phenotypes of a mutant
reduced stationary phase mutagenesis PubMed
a uvrA uvrB mutant is sensitive to blue light-induced DNA damage PubMed
sensitive to Cr(VI) treatment PubMed
reduced resistance towards electron beams PubMed
The protein
Protein family
ABC transporter superfamily (according to UniProt)
Domains
2 ABC transporter domains (aa 309-587, aa 607-935) (according to UniProt)
Structure
2R6F (PDB) (Geobacillus stearothermophilus)
O34863 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
uvrB-uvrA
Description
PubMed
Regulation
induced upon DNA damage (LexA) PubMed
Regulatory mechanism
LexA: repression, PubMed, in lexA regulon
Additional information
during sporulation expressed both in the forespore and the mother cell PubMed
expression is induced in the presence of Cr(VI) PubMed
Open in new tab

uvrB→uvrA

2025-01-14 22:41:07

Jstuelk

87

64e9c2eef7258297470790f72dba7931d9d59a23

8F26139F6B9D375FE6C35423921E79A506C28EB3

Biological materials
Mutant
GP3534 (Δ(uvrA-uvrB)::kan), available in Jörg Stülke's lab
GP1175 (Δ(uvrA-uvrB)::ermC), available in Jörg Stülke's lab PubMed
BKE35160 (uvrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TATCCGATCCATAGCCATTT,  downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG
BKK35160 (uvrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TATCCGATCCATAGCCATTT,  downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG
References
Reviews
Loading
Original Publications
Loading

39B73AADBFD42203ADFA2E834697799E6430A086

Page visits: 3022

Time of last update: 2025-04-03 15:46:18

Author of last update: Jstuelk