rhiL

rhiL
168

ABC transporter (sugar-binding protein) for rhamnose oligosaccharides

Locus
BSU_06970
Molecular weight
46.45 kDa
Isoelectric point
4.99
Protein length
427 aaSequenceBlast
Gene length
1284 bpSequenceBlast
Function
uptake of rhamnose oligosaccharides
Product
rhamnose oligosaccharide ABC transporter
Essential
no
Synonyms
rhiL, yesO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
761,662  762,945
The protein
Protein family
Bacterial solute-binding protein 1 family (according to UniProt)
Structure
4R6K (PDB)
5Z6B (PDB) (complexed with rhamnogalacturonan trisaccharide) PubMed
5Z6C (PDB) (complexed with rhamnogalacturonan) PubMed
O31518 (AlphaFold)
Localization
associated to the membrane (via RhiF-RhiG) PubMed
Expression and Regulation
Operons
Genes
rhiL-rhiF-rhiG-rhiN-rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ
Description
PubMed
Regulation
induced by pectin (RhgR, RhgL) PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
RhgR: activation, PubMed, in rhgR regulon
SigA: Sigma factor, PubMed, in sigA regulon
RhgL: activation, PubMed, in rhgL regulon
CcpA: repression, PubMed, in ccpA regulon
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rhiL→yesZ

2025-03-16 17:43:25

Jstuelk

117

D891C8F38E150B653BA655BA6352352D86D18BAE

A10D4124F5AF6831808648D33C98AF061D3703AA

Biological materials
Mutant
MGNA-A947 (yesO::erm), available at the NBRP B. subtilis, Japan
BKE06970 (rhiL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAACCAATCCCCCCTTTAA,  downstream forward: _UP4_AATGAGATATTAGAGAGGAA
BKK06970 (rhiL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAACCAATCCCCCCTTTAA,  downstream forward: _UP4_AATGAGATATTAGAGAGGAA
References
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A96A83684E1E6E8705265D3932DA60FF47C23E3B

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Time of last update: 2025-04-04 12:27:49

Author of last update: Jstuelk