rhiL

rhiL
168

ABC transporter (sugar-binding protein) for rhamnose oligosaccharides

Locus
BSU_06970
Molecular weight
46.45 kDa
Isoelectric point
4.99
Protein length
427 aaSequenceBlast
Gene length
1284 bpSequenceBlast
Function
uptake of rhamnose oligosaccharides
Product
rhamnose oligosaccharide ABC transporter
Essential
no
Synonyms
rhiL, yesO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
761,662  762,945
The protein
Protein family
Bacterial solute-binding protein 1 family (according to UniProt)
Structure
4R6K (PDB)
5Z6B (PDB) (complexed with rhamnogalacturonan trisaccharide) PubMed
5Z6C (PDB) (complexed with rhamnogalacturonan) PubMed
O31518 (AlphaFold)
Localization
associated to the membrane (via RhiF-RhiG) PubMed
Expression and Regulation
Operons
Genes
rhiL-rhiF-rhiG-rhiN-rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ
Description
PubMed
Regulation
induced by pectin (RhgR, RhgL) PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
RhgR: activation, PubMed, in rhgR regulon
SigA: Sigma factor, PubMed, in sigA regulon
RhgL: activation, PubMed, in rhgL regulon
CcpA: repression, PubMed, in ccpA regulon
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rhiL→yesZ

2025-03-16 17:43:25

Jstuelk

117

D891C8F38E150B653BA655BA6352352D86D18BAE

A10D4124F5AF6831808648D33C98AF061D3703AA

Biological materials
Mutant
MGNA-A947 (yesO::erm), available at the NBRP B. subtilis, Japan
BKE06970 (rhiL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAACCAATCCCCCCTTTAA,  downstream forward: _UP4_AATGAGATATTAGAGAGGAA
BKK06970 (rhiL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAACCAATCCCCCCTTTAA,  downstream forward: _UP4_AATGAGATATTAGAGAGGAA
References
Mahendran A, Orlando BJGenome wide structural prediction of ABC transporter systems in Bacillus subtilis.Frontiers in microbiology. 2024; 15:1469915. PMID: 39397791
O'Reilly FJ, Graziadei A, Forbrig C, Bremenkamp R, Charles K, Lenz S, Elfmann C, Fischer L, Stülke J, Rappsilber JProtein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Hirooka KRhgKL and CcpA directly regulate the rhiLFGN-rhgR-yesTUV operon involved in the metabolism of rhamnogalacturonan type I in Bacillus subtilis.Bioscience, biotechnology, and biochemistry. 2022 Jul 26; . PMID: 35881471
Sugiura H, Nagase A, Oiki S, Mikami B, Watanabe D, Hashimoto WBacterial inducible expression of plant cell wall-binding protein YesO through conflict between Glycine max and saprophytic Bacillus subtilis.Scientific reports. 2020 Oct 29; 10(1):18691. PMID: 33122638
Rodionova IA, Li X, Thiel V, Stolyar S, Stanton K, Fredrickson JK, Bryant DA, Osterman AL, Best AA, Rodionov DA Comparative genomics and functional analysis of rhamnose catabolic pathways and regulons in bacteria. Frontiers in microbiology. 2013; 4:407. doi:10.3389/fmicb.2013.00407. PMID:24391637
Ochiai A, Itoh T, Kawamata A, Hashimoto W, Murata K Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization. Applied and environmental microbiology. 2007 Jun; 73(12):3803-13. . PMID:17449691
Quentin Y, Fichant G, Denizot F Inventory, assembly and analysis of Bacillus subtilis ABC transport systems. Journal of molecular biology. 1999 Apr 02; 287(3):467-84. . PMID:10092453

A96A83684E1E6E8705265D3932DA60FF47C23E3B

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Time of last update: 2025-04-04 12:29:54

Author of last update: Jstuelk