pdxS
168

pyridoxal-5-phosphate synthase (synthase domain)

Locus

BSU_00110

Molecular weight

31.46 kDa

Isoelectric point

5.09

Protein length

294 aaSequenceBlast

Gene length

885 bpSequenceBlast

Function

pyridoxal-5-phosphate biosynthesis

Product

pyridoxal-5-phosphate synthase (synthase domain)

Essential

E.C.

4.3.3.6

Synonyms

pdxS, yaaD

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0214 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

19,062 19,946

Phenotypes of a mutant

the mutant tends to acquire suppressor mutations that result in improved growth and colony morphology PubMed

auxotrophic for pyridoxal 5'-phosphate PubMed

inactivation of pdxS reduces sporulation efficiency to 1.2% that of wild type cells PubMed

poor growth PubMed

non-transformable PubMed

The protein

Catalyzed reaction/ biological activity

Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. (according to UniProt)

aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine --> H⁺ + 3 H₂O + L-glutamate + phosphate + pyridoxal 5'-phosphate (according to UniProt)

Protein family

PdxS/SNZ family (single member, according to UniProt)

Structure

2NV2 (PDB) PdxS-PdxT complex PubMed

2NV1 (PDB) PdxS PubMed

P37527 (AlphaFold)

Modification

phosphorylated on Arg-8 PubMed

phosphorylated on STY PubMed, PubMed

Additional information

belongs to the 100 most abundant proteins PubMed

Expression and Regulation