flhA

flhA
168

part of the flagellar type III export apparatus (flagellar Type III secretion system), part of the CORE complex required for flagellum and nanotube assembly

Locus
BSU_16390
Molecular weight
73.77 kDa
Isoelectric point
4.64
Protein length
677 aaSequenceBlast
Gene length
2034 bpSequenceBlast
Function
flagellum and nanotube assembly
Product
part of the type III CORE export apparatus
Essential
no
Synonyms
flhA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1298 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,707,714  1,709,747
Phenotypes of a mutant
no secretion of FlgM, permanent inhibition of SigD PubMed
inactivation of ''flhA'' confers resistance to high concentrations of Zn(II) PubMed
deficient in both nanotube production and the associated intercellular molecular trafficking PubMed
defective in Swarming motility PubMed
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C PubMed
The protein
Protein family
FHIPEP (flagella/HR/invasion proteins export pore) family (single member, according to UniProt)
Structure
3MIX (PDB) (cytosolic domain) PubMed
P35620 (AlphaFold)
Localization
cell membrane PubMed, 8 transmembrane helices PubMed
Expression and Regulation
Operons
Genes
ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
see fla-che operon
Regulatory mechanism
Spo0A: repression, in spo0A regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SwrA: activation, in swrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

ylxF→swrB

2025-04-04 21:21:07

Jstuelk

170

EECFE9022742ECC57D9BBA5CE7DB7A4D1BFD42A8

0535E3D13CBD000A94D62050515BF8D1D5E3F011

Genes
flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
Spo0A: repression, in spo0A regulon
SwrA: activation, in swrA regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

flgB→swrB

2025-04-03 01:56:48

Jstuelk

173

024d59ae468aa14568753f57122b789c39ed6c06

4D920B925DA071AE7390E022B9AB625E40B7B2AC

Biological materials
Mutant
BKE16390 (flhA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGTTTTTTCTCTCCT,  downstream forward: _UP4_AGCATTGGAGTGGTGGATAT
BKK16390 (flhA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGTTTTTTCTCTCCT,  downstream forward: _UP4_AGCATTGGAGTGGTGGATAT
References
Reviews
Altegoer F, Bange G Undiscovered regions on the molecular landscape of flagellar assembly. Current opinion in microbiology. 2015 Dec; 28:98-105. doi:10.1016/j.mib.2015.08.011. pii:S1369-5274(15)00138-1. PMID:26490009
Minamino T Protein export through the bacterial flagellar type III export pathway. Biochimica et biophysica acta. 2014 Aug; 1843(8):1642-8. doi:10.1016/j.bbamcr.2013.09.005. pii:S0167-4889(13)00327-3. PMID:24064315
Minamino T, Imada K, Namba K Mechanisms of type III protein export for bacterial flagellar assembly. Molecular bioSystems. 2008 Nov; 4(11):1105-15. doi:10.1039/b808065h. PMID:18931786
Original Publications
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Time of last update: 2025-04-09 20:55:42

Author of last update: Jstuelk