fliO

fliO
168

part of the flagellar type III export apparatus (flagellar Type III secretion system), part of the CORE complex required for flagellum and nanotube assembly

Locus
BSU_16340
Molecular weight
24.72 kDa
Isoelectric point
9.33
Protein length
219 aaSequenceBlast
Gene length
660 bpSequenceBlast
Function
flagellum and nanotube assembly
Product
part of the type III CORE export apparatus
Essential
no
Synonyms
fliZ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3190 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,704,211 → 1,704,870
Phenotypes of a mutant
no secretion of FlgM, permanent inhibition of SigD PubMed
deficient in both nanotube production and the associated intercellular molecular trafficking PubMed
defective in Swarming motility PubMed
The protein
Structure
P35536 (AlphaFold)
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
see fla-che operon
Regulatory mechanism
Spo0A: repression, in spo0A regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SwrA: activation, in swrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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ylxF→swrB

2025-04-04 21:21:07

Jstuelk

170

EECFE9022742ECC57D9BBA5CE7DB7A4D1BFD42A8

0535E3D13CBD000A94D62050515BF8D1D5E3F011

Genes
flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
Spo0A: repression, in spo0A regulon
SwrA: activation, in swrA regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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flgB→swrB

2025-04-03 01:56:48

Jstuelk

173

024d59ae468aa14568753f57122b789c39ed6c06

4D920B925DA071AE7390E022B9AB625E40B7B2AC

Biological materials
Mutant
BKE16340 (ΔfliO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTTTTCAACAGTCGTACAC,  downstream forward: _UP4_GGAAAGAAGAAAGGCCCACG
BKK16340 (ΔfliO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTTTTCAACAGTCGTACAC,  downstream forward: _UP4_GGAAAGAAGAAAGGCCCACG
References
Reviews
Altegoer F, Bange G Undiscovered regions on the molecular landscape of flagellar assembly. Current opinion in microbiology. 2015 Dec; 28:98-105. doi:10.1016/j.mib.2015.08.011. pii:S1369-5274(15)00138-1. PMID:26490009
Original Publications
Sanchez S, Snider EV, Wang X, Kearns DBIdentification of Genes Required for Swarming Motility in Bacillus subtilis Using Transposon Mutagenesis and High-Throughput Sequencing (TnSeq).Journal of bacteriology. 2022 Jun 21; 204(6):e0008922. PMID: 35638827
Bhattacharya S, Baidya AK, Pal RR, Mamou G, Gatt YE, Margalit H, Rosenshine I, Ben-Yehuda S A Ubiquitous Platform for Bacterial Nanotube Biogenesis. Cell reports. 2019 Apr 09; 27(2):334-342.e10. pii:S2211-1247(19)30234-7. doi:10.1016/j.celrep.2019.02.055. PMID:30929979
Calvo RA, Kearns DB FlgM is secreted by the flagellar export apparatus in Bacillus subtilis. Journal of bacteriology. 2015 Jan 01; 197(1):81-91. doi:10.1128/JB.02324-14. PMID:25313396
Mordini S, Osera C, Marini S, Scavone F, Bellazzi R, Galizzi A, Calvio C The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. PloS one. 2013; 8(12):e85065. doi:10.1371/journal.pone.0085065. PMID:24386445
Marchadier E, Carballido-López R, Brinster S, Fabret C, Mervelet P, Bessières P, Noirot-Gros MF, Fromion V, Noirot P An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach. Proteomics. 2011 Aug; 11(15):2981-91. doi:10.1002/pmic.201000791. PMID:21630458
Kobayashi K Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis. Molecular microbiology. 2007 Oct; 66(2):395-409. . PMID:17850253
Werhane H, Lopez P, Mendel M, Zimmer M, Ordal GW, Márquez-Magaña LM The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function. Journal of bacteriology. 2004 Jun; 186(12):4025-9. . PMID:15175317
Molle V, Fujita M, Jensen ST, Eichenberger P, González-Pastor JE, Liu JS, Losick R The Spo0A regulon of Bacillus subtilis. Molecular microbiology. 2003 Dec; 50(5):1683-701. . PMID:14651647
Estacio W, Anna-Arriola SS, Adedipe M, Márquez-Magaña LM Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis. Journal of bacteriology. 1998 Jul; 180(14):3548-55. . PMID:9657996
Márquez-Magaña LM, Chamberlin MJ Characterization of the sigD transcription unit of Bacillus subtilis. Journal of bacteriology. 1994 Apr; 176(8):2427-34. . PMID:8157612
Bischoff DS, Weinreich MD, Ordal GW Nucleotide sequences of Bacillus subtilis flagellar biosynthetic genes fliP and fliQ and identification of a novel flagellar gene, fliZ. Journal of bacteriology. 1992 Jun; 174(12):4017-25. . PMID:1597417

C2C67880EDF09E1BA75A4791628EE1982F9B6B0B

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Time of last update: 2025-04-11 11:44:42

Author of last update: Jstuelk