phoA

phoA
168

alkaline phosphatase A

Locus
BSU_09410
Molecular weight
50.11 kDa
Isoelectric point
9.93
Protein length
461 aaSequenceBlast
Gene length
1386 bpSequenceBlast
Function
aquisition of phosphate upon phosphate starvation
Product
alkaline phosphatase A
Essential
no
E.C.
3.1.3.1
Synonyms
phoA, phoAIV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1785 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,017,083 → 1,018,468
The protein
Catalyzed reaction/ biological activity
phosphate monoester + H2O --> alcohol + phosphate (according to UniProt)
Protein family
alkaline phosphatase family (with PhoB, according to UniProt)
Structure
3A52 (PDB) (from Shewanella sp., mature protein without signal peptide, 40% identity) PubMed
P19406 (AlphaFold)
Paralogous protein(s)
PhoB
Localization
extracellular (signal peptide) PubMed
Expression and Regulation
Operons
Genes
phoA
Description
PubMed
Regulation
expressed under conditions of phosphate limitation (PhoP) PubMed
Regulatory mechanism
PhoP: activation, PubMed, in phoP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigG: sigma factor, PubMed, in sigG regulon
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phoA

2025-03-19 17:36:34

ghost

79

c5f909c79db9c85c38e7128cd5bf36305bf1c419

179175A7CC709AA58606A1C79DFA45FC4CC334EF

Biological materials
Mutant
1A767 ( phoA::cat), PubMed, available at BGSC
BKE09410 (ΔphoA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCTCCTTTAATGTATT,  downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
BKK09410 (ΔphoA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCTCCTTTAATGTATT,  downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
Labs working on this gene/protein
Marion Hulett, University of Illinois at Chicago, USA Homepage
References
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E0430C16C41440633647976A0F9183E24268E198

Page visits: 7658

Time of last update: 2025-04-10 12:35:10

Author of last update: Melvin.boenninger