sasB

sasB
168

(p)ppGpp synthetase, contributes to cellular heterogeneity in protein synthesis during nutrient limitation

Locus
BSU_11600
Molecular weight
24.56 kDa
Isoelectric point
6.71
Protein length
211 aaSequenceBlast
Gene length
636 bpSequenceBlast
Function
ppGpp synthesis independent from stringent response
Product
(p)ppGpp synthetase
Essential
no
Synonyms
sasB, yjbM, relQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2357 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,237,006  1,237,641
Phenotypes of a mutant
a rel sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
a rel sasA sasB triple mutant acquires suppressor mutations in guaA, guaB, gmk or inactivation of codY PubMed
a rel sasA sasB triple mutant is unable to grow in the presence of guanosine, this can be suppressed by inactivation of pyk, hprT, or gmk PubMed
The protein
Catalyzed reaction/ biological activity
ATP + GTP --> AMP + guanosine 3'-diphosphate 5'-triphosphate (according to UniProt)
Protein family
RelA/SpoT family (with SasA and Rel, according to UniProt)
Domains
single ppGpp synthetase domain PubMed
Structure
5DEC (PDB) PubMed
O31611 (AlphaFold)
Effectors of protein activity
allosterically activated by pppGpp PubMed, this is inhibited by the SasA product pGpp PubMed
Paralogous protein(s)
SasA
Expression and Regulation
Operons
Genes
yjbL-sasB-nadF-yjbO
Description
PubMed
Regulation
expressed during exponential growth
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yjbL→yjbO

2025-04-03 22:19:01

Jstuelk

101

c5edd61e714281007d4d9ec99a1efa0cfef63f09

83E46551CBC32AB07130281546FE3D422612C2C3

Biological materials
Mutant
MGNA-B159 (yjbM::erm), available at the NBRP B. subtilis, Japan
GP2066 (sasA sasB rel::mls), available in Jörg Stülke's lab
BKE11600 (sasB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATACATCCCCCAATTC,  downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
BKK11600 (sasB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATACATCCCCCAATTC,  downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
GP3470 (ΔsasA::spec ΔsasB::phleo), available in Jörg Stülke's lab
GP3472 (Δrel::cat ΔsasB::phleo), available in Jörg Stülke's lab
GP3425 (ΔsasB::phleo) , available in Jörg Stülke's lab
GP3467 (sasB-6xHis-cat), available in Jörg Stülke's lab
Expression vectors
pGP3452 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
LacZ fusion
GP3693 (based on pAC7), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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81BCF5C08EC1A580F5E05884BA830130FC7E46E9

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Time of last update: 2025-04-06 01:50:09

Author of last update: Jstuelk