ScpB

• Description: part of the condensin complex, chromosomal origin condensation and segregation
  
  

Gene name	scpB
Synonyms	ypuH
Essential	yes
Product	DNA segregation and condensation protein
Function	segregation of replication origins
Gene expression levels in SubtiExpress: scpB
Interactions involving this protein in SubtInteract: ScpB
MW, pI	21 kDa, 4.25
Gene length, protein length	591 bp, 197 aa
Immediate neighbours	ypuI, scpA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU23210

Phenotypes of a mutant

• scpB mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed

Database entries

• BsubCyc: BSU23210

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: scpB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • part of the condensin complex PubMed
  • forms dimers PubMed
  • ScpB-Smc PubMed, this interaction takes place only in the presence of ScpA PubMed
  • ScpA-ScpB PubMed

• Localization:
  • nucleoid (Multiple) PubMed
  • the condensin (Smc)2-ScpA-ScpB complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on Spo0J PubMed

Database entries

• BsubCyc: BSU23210

• Structure:
  • 4I98 (complex between ScpA (aa 1-160)-ScpB (aa 1-183) PubMed

• UniProt: P35155

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: scpA-scpB-ypuI PubMed

• Expression browser: scpB PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Sophie Nolivos, David Sherratt
The bacterial chromosome: architecture and action of bacterial SMC and SMC-like complexes.
FEMS Microbiol Rev: 2014, 38(3);380-92
[PubMed:24118085] [WorldCat.org] [DOI] (I p)

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)