sucC

sucC
168

succinyl-CoA synthetase (beta subunit)

Locus
BSU_16090
Molecular weight
41.21 kDa
Isoelectric point
4.85
Protein length
385 aaSequenceBlast
Gene length
1158 bpSequenceBlast
Function
TCA cycle
Product
succinyl-CoA synthetase (beta subunit)
Essential
no
E.C.
6.2.1.5
Synonyms
sucC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0045 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,680,431 1,681,588
The protein
Catalyzed reaction/ biological activity
ATP + CoA + succinate --> ADP + phosphate + succinyl-CoA (according to UniProt)
Protein family
succinate/malate CoA ligase beta subunit family (single member, according to UniProt)
Domains
ATP-grasp domain (aa 9-244) (according to UniProt)
Structure
1JKJ (PDB) (E. coli)
P80886 (AlphaFold)
Modification
phosphorylation on Ser-220 PubMed
Effectors of protein activity
Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
GTP is not accepted by the enzyme FEBS Letters
Kinetic information
Reversible Michaelis-Menten FEBS Letters
Additional information
extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
sucC-sucD-dprA-topA
Description
PubMed
Regulation
repressed by glucose (2.4-fold) (CcpA) PubMed
expression is heterogeneous PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
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sucC→topA

2025-03-17 21:02:13

Jstuelk

93

167fd8fae94fab138af8eabe6101bc58adfb0921

2BC79D48E345B722DDAE396E448CF1DB3AAB049C

Other regulations
RoxS: translation inhibition, translation inhibition and initiation of RNA degradation by Rnc
Biological materials
Mutant
1A1006 sucC::spec, available at BGSC
GP1134 (cat), available in Jrg Stlke's lab
GP791 (sucC-sucD)::tet, available in Jrg Stlke's lab
GP2344 (sucC-sucD)::kan, Cre-recombinase is integrated in sacA, available in Jrg Stlke's lab
GP2345 (sucC-sucD::lox72), Cre-recombinase is integrated in sacA, available in Jrg Stlke's lab
BKE16090 (sucC)::erm trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCCATCCTCCTAACT, downstream forward: _UP4_TAAGAAAGAATGAAAGGCAG
BKK16090 (sucC)::kan trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCCATCCTCCTAACT, downstream forward: _UP4_TAAGAAAGAATGAAAGGCAG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab
References
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Time of last update: 2025-04-13 11:42:41

Author of last update: Jstuelk