aprE

aprE
168

major extracellular alkaline protease

Locus
BSU_10300
Molecular weight
39.37 kDa
Isoelectric point
9.34
Protein length
381 aaSequenceBlast
Gene length
1146 bpSequenceBlast
Function
protein degradation
Product
extracellular alkaline serine protease (subtilisin E)
Essential
no
Synonyms
aprE, sprE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,104,423  1,105,568
Phenotypes of a mutant
impaired biofilm architecture PubMed
The protein
Catalyzed reaction/ biological activity
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides(according to UniProt)
Protein family
peptidase S8 family (according to UniProt)
Domains
Inhibitor I9 domain (aa 38-109) (according to UniProt)
Peptidase S8 domain (aa 111-380) (according to UniProt)
Structure
1SBC (PDB)
P04189 (AlphaFold)
Localization
secreted (according to Swiss-Prot),  extracellular (signal peptide) PubMed
Expression and Regulation
Operons
Genes
aprE
Description
PubMed
Regulation
repressed by SinR PubMed
induced in the presence of external ComX PubMed
Regulatory mechanism
SinR: repression, PubMed, in sinR regulon
ScoC: repression, PubMed, in scoC regulon
AbrB: repression, PubMed, in abrB regulon
DegU: activation, DegU-P, PubMed, in degU regulon
CodY: repression, PubMed, in codY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
in a triple ''abrB codY scoC'' mutant, expression occurs during exponential growth PubMed
Open in new tab

aprE

2025-03-15 16:56:09

Jstuelk

87

121ffe70e800e6a61942dad99d4adbe1a7d0d708

5C777B5A576DEF3DFD930F88AF6E3E8269A0737C

Additional information
expression is increased in a hag mutant due to the presence of increased levels of DegU-P PubMed
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
KO7 (nprE  aprE  epr  mpr  nprB  vpr  bpr), available as BGSC 1A1133
BKE10300 (aprE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT,  downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG
BKK10300 (aprE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT,  downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG
Antibody
**
References
Reviews
Loading
Original Publications
Loading

0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D

Page visits: 15463

Time of last update: 2025-04-07 06:01:02

Author of last update: Jstuelk