odhA

odhA
168

2-oxoglutarate dehydrogenase (E1 subunit)

Locus
BSU_19370
Molecular weight
105.54 kDa
Isoelectric point
5.87
Protein length
944 aaSequenceBlast
Gene length
2835 bpSequenceBlast
Function
TCA cycle
Product
2-oxoglutarate dehydrogenase (E1 subunit)
Essential
no
E.C.
1.2.4.2
Synonyms
odhA, citK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0567 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,108,774  2,111,608
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase]-(R)-N6-lipoyl-L-lysine + H+ --> [dihydrolipoyllysine-residue succinyltransferase]-(R)-N6-(S8-succinyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
Protein family
alpha-ketoglutarate dehydrogenase family (single member, according to UniProt)
Cofactors
TPP PubMed
Structure
2JGD (PDB) (from Escherichia coli, 38% identity, 55% similarity) PubMed
P23129 (AlphaFold)
Modification
phosphorylated on Arg-66 and Arg-621 PubMed
Localization
cytoplasm (according to UniProt)
Additional information
extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia
Expression and Regulation
Operons
Genes
odhA-odhB
Description
PubMed
Regulation
repressed by glucose (2.4-fold) (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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odhA→odhB

2025-04-09 14:09:07

ghost

104

998628ac6a5b555702d5fa0febcedb0378ac763b

87DE9AE10510310314B50140AE66E41B4448BC3A

Biological materials
Mutant
GP671 (spc), GP684 (cat), available in Jörg Stülke's lab
GP1274 Δ(odhA)::cat, available in Jörg Stülke's lab
GP1276 Δ(odhA-odhB)::cat, available in Jörg Stülke's lab
GP2183 Δ(odhA)::ermC, available in Jörg Stülke's lab PubMed
GP2332 Δ(odhA-odhB)::kan, Cre-recombinase is integrated in ''sacA'', available in Jörg Stülke's lab
GP2334 Δ(odhA-odhB)::lox72, Cre-recombinase is integrated in ''sacA'', available in Jörg Stülke's lab
BKE19370 (odhA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGAATATTACCCCCAA,  downstream forward: _UP4_AAAAACTAAGGGGGAAATGA
BKK19370 (odhA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGAATATTACCCCCAA,  downstream forward: _UP4_AAAAACTAAGGGGGAAATGA
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
LacZ fusion
pGP3106 (in pAC7), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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3B54AD8514709655BA829347B7BD5B38EF7C08ED

Page visits: 5299

Time of last update: 2025-04-10 17:02:56

Author of last update: Jstuelk