sufS
168

cysteine desulfurase, cysteine:SufU sulfuryl transferase

Locus

BSU_32690

Molecular weight

44.76 kDa

Isoelectric point

5.2

Protein length

406 aaSequenceBlast

Gene length

1221 bpSequenceBlast

Function

formation of iron-sulfur clusters in proteins

Product

cysteine desulfurase

Essential

yes

E.C.

2.8.1.7

Synonyms

sufS, yurW, csd

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0520 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,357,444 3,358,664

Phenotypes of a mutant

essential PubMed

terminal phenotype of a CRISPRi knockdown mutant is moderate bulging PubMed

the mutant is viable and Fe-S clusters are dispensable if IspG and IspH from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate PubMed

The protein

Catalyzed reaction/ biological activity

transfer of iron to SufU PubMed

[sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)

Protein family

Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

pyridoxalphosphate PubMed

Structure

5J8Q (PDB) PubMed

5XT5 (PDB) (the SufS₂-SufU₂ complex) PubMed

5ZS9 (PDB) (resting state)