yabA
168

inhibitor of DnaA oligomerization, tethers DnaA to the polymerase-clamp protein DnaN

Locus

BSU_00330

Molecular weight

13.96 kDa

Isoelectric point

6.07

Protein length

119 aaSequenceBlast

Gene length

360 bpSequenceBlast

Function

positioning of DnaA

Product

negative regulator of replication initiation

Essential

Synonyms

yabA

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4467 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

42,499 42,858

Phenotypes of a mutant

strongly reduced growth rate in minimal medium PubMed

over-initiation of replication PubMed

a whiA yabA double mutant is not viable PubMed

synthetically lethal in combination with a dnaA1 mutation, this can be suppressed by mutations in rel or nrdR that result in stimulated replication elongation, by mutations in dnaC or cshA that result in decreased replication initiation as well as by inactivation of ccrZ PubMed

The protein

Catalyzed reaction/ biological activity

negative control of replication initiation PubMed

associates with the oriC region in a DnaA-dependent manner, this limits the amount of DnaA that can bind the oriC region PubMed

YabA inhibits co-operative DNA binding by DnaA PubMed

inhibits oligomerization and helix formation of DnaA PubMed

Protein family

YabA family (single member, according to UniProt)

Domains

leucine zipper

Cofactors

Zinc PubMed