Difference between revisions of "DarA"
| Line 4: | Line 4: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''darA'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yaaQ '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || | |style="background:#ABCDEF;" align="center"| '''Essential''' || | ||
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown | |style="background:#ABCDEF;" align="center"|'''Function''' || unknown | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00290 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00290 darA] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 6.088 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 6.088 | ||
| Line 103: | Line 103: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[yaaQ]]-[[ | + | * '''Operon:''' ''[[yaaQ]]-[[darA]]-[[holB]]-[[yaaT]]-[[yabA]]-[[yabB]]-[[yazA]]-[[yabC]]'' {{PubMed|22383849}} |
| − | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yaaQ_39871_40200_1 | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yaaQ_39871_40200_1 darA] {{PubMed|22383849}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| Line 120: | Line 120: | ||
=Biological materials = | =Biological materials = | ||
* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP1712 (''[[ | + | ** GP1712 (''[[darA]]''::''cat''), available in [[Jörg Stülke]]'s lab |
| − | ** GP1713 (''[[ | + | ** GP1713 (''[[darA]]-[[yaaR]]''::''cat''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
Revision as of 12:55, 10 November 2014
- Description: unknown
| Gene name | darA |
| Synonyms | yaaQ |
| Essential | |
| Product | unknown |
| Function | unknown |
| Gene expression levels in SubtiExpress: darA | |
| MW, pI | 11 kDa, 6.088 |
| Gene length, protein length | 327 bp, 109 aa |
| Immediate neighbours | tmk, yaaR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00290
Phenotypes of a mutant
Database entries
- BsubCyc: BSU00290
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Interactions:
- forms homo-trimers (see 3M05)
Database entries
- BsubCyc: BSU00290
- Structure:
- 3M05 (the protein from Pediococcus pentosaceus, 50% identity)
- UniProt: P37538
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1030 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 922 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 894 PubMed
Biological materials
- Mutant:
- GP1712 (darA::cat), available in Jörg Stülke's lab
- GP1713 (darA-yaaR::cat), available in Jörg Stülke's lab
- Expression vector:
- pGP2601: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
- pGP2624: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
- pGP2603: expression of yaaQ-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody: available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References
