dctS

dctS
168

two-component sensor kinase, regulation of the dctS-dctR-dctP operon

Locus
BSU_04450
Molecular weight
59.77 kDa
Isoelectric point
6.2
Protein length
535 aaSequenceBlast
Gene length
1608 bpSequenceBlast
Function
regulation of the dctS-dctR-dctP operon
Product
two-component sensor kinase
Essential
no
Synonyms
dctS, ydbF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3290 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
497,768  499,375
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of DctR
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
Domains
two transmembrane segments
PAS domain (aa 213–276) (according to UniProt)
Histidine kinase domain (aa 333-528) (according to UniProt)
Structure
4I5S (PDB) (from Streptococcus mutans, corresponds to aa 199 ... 529, 22% identity) PubMed
P96601 (AlphaFold)
Modification
autophosphorylation on a His residue
Effectors of protein activity
DctB and DctP act as co-sensors with DctS PubMed
Localization
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
dctS-dctR-dctP
Description
PubMed
Regulation
part of the iron sparing response, strong down-regulation in a'' fur'' mutant (Fur, FsrA) PubMed
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dctS→dctP

2025-04-02 07:02:32

ghost

101

4dd03fff83218f17096547a66eb1524319299251

D88BB436513B36F213FD5665E04DA7A2C0B76593

Biological materials
Mutant
GP225 (dctS::cat), available in Jörg Stülke's lab
BKE04450 (dctS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGCTTCTCCTTTCAGG,  downstream forward: _UP4_CCAATGAAGGGGGAGGAAGC
BKK04450 (dctS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGCTTCTCCTTTCAGG,  downstream forward: _UP4_CCAATGAAGGGGGAGGAAGC
References
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9A96572B101358768EB20EE9438BE0E1424A4130

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Time of last update: 2025-04-06 14:31:26

Author of last update: Jstuelk