dgcW

dgcW
168

diguanylate cyclase and potential phosphodiesterase

Locus
BSU_13420
Molecular weight
84.63 kDa
Isoelectric point
6.82
Protein length
749 aaSequenceBlast
Gene length
2250 bpSequenceBlast
Function
synthesis of c-di-GMP
Product
diguanylate cyclase and potential phosphodiesterase
Essential
no
Synonyms
dgcW, ykoW
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG3300 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,407,329  1,409,578
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-GMP from two molecules of GTP PubMed
Domains
MHYT domain (aa 7-201) (according to UniProt)
contains a N-terminal PAS domain PubMed
contains a central GGDEF domain and a C-terminal EAL domain PubMed
aa 290-735 are similar to E.  coli CsrD (18% identity, 43% similarity)
Structure
5XGB (PDB) (from Pseudomonas aeruginosa, corresponds to the C-terminal part of DgcW, aa 194 ... 735, 29% identity) PubMed
O34311 (AlphaFold)
Localization
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
dgcW
Description
PubMed
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
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dgcW

2025-03-18 06:51:28

ghost

76

3e750b0ff0745774be04b232f89ddb7bd76cf215

D6074E82B5ABD2EBA34CE3DEF4860CF155B238CC

Biological materials
Mutant
MGNA-B314 (ykoW::erm), available at the NBRP B. subtilis, Japan
BP140 (''dgcW::ermC'') available in Fabian Commichau's lab
BP142 (''dgcW-ykoV-ligD::kan'') available in Fabian Commichau's lab
BKE13420 (dgcW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT,  downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT
BKK13420 (dgcW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT,  downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
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Time of last update: 2025-04-06 12:05:54

Author of last update: Jstuelk