Difference between revisions of "WalK"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
+ | ** pGP2636, constitutive expression in ''B. subtilis'' (based on [[pBQ200]]), available in [[Jörg Stülke]]'s lab | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' |
Revision as of 13:51, 24 September 2014
- Description: two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors
Gene name | walK |
Synonyms | yycG |
Essential | yes PubMed |
Product | two-component sensor kinase (OmpR family) |
Function | control of cell wall metabolism |
Gene expression levels in SubtiExpress: walK | |
Interactions involving this protein in SubtInteract: WalK | |
MW, pI | 69 kDa, 4.775 |
Gene length, protein length | 1833 bp, 611 aa |
Immediate neighbours | yycH, walR |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell wall/ other, protein modification, transcription factors and their control, essential genes, membrane proteins, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU40400
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU40400
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of WalR
- Protein family: two-component sensor kinase of the OmpR family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
- Modification: autophosphorylation on a His residue
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU40400
- UniProt: Q45614
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: expressed during vegetative growth, repressed during stationary phase PubMed
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (complex medium with amino acids, without glucose): 236 PubMed
Biological materials
- Mutant:
- Expression vector:
- pGP2636, constitutive expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Publications