lmrA

lmrA
168

transcriptional regulator (TetR family), control of qdoI-yxaH and lmrA-lmrB

Locus
BSU_02680
Molecular weight
20.52 kDa
Isoelectric point
5.03
Protein length
188 aaSequenceBlast
Gene length
567 bpSequenceBlast
Function
regulation of lincomycin resistance
Product
transcriptional regulator
Essential
no
Synonyms
lmrA, lin-2, yccB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1309 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
290,132  290,698
The protein
Protein family
TetR family
Domains
HTH tetR-type domain (aa 4-64) (according to UniProt)
Structure
1SGM (PDB) (QdoR, 56% identity) PubMed
O34619 (AlphaFold)
Effectors of protein activity
flavonoids such as quercetin serve as inducers, binding results in release from DNA PubMed
Paralogous protein(s)
QdoR
Expression and Regulation
Operons
Genes
lmrA-lmrB
Description
PubMed
Regulation
induced by flavonoids such as quercetin (LmrA)PubMed
Regulatory mechanism
LmrA: repression, PubMed, in lmrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
Open in new tab

lmrA→lmrB

2025-04-06 20:43:13

ghost

114

be7795d98f26be96a984dc95802c21f2dcb1e545

FCA0758D41C878E1059BF369D1C30C97AACB6B0C

Biological materials
Mutant
BKE02680 (lmrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACATTCCCACCTTACT,  downstream forward: _UP4_TAAAAAAAACGACATACTAC
BKK02680 (lmrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACATTCCCACCTTACT,  downstream forward: _UP4_TAAAAAAAACGACATACTAC
References
Reviews
Hirooka K Transcriptional response machineries of Bacillus subtilis conducive to plant growth promotion. Bioscience, biotechnology, and biochemistry. 2014; 78(9):1471-84. doi:10.1080/09168451.2014.943689. PMID:25209494
Cuthbertson L, Nodwell JR The TetR family of regulators. Microbiology and molecular biology reviews : MMBR. 2013 Sep; 77(3):440-75. doi:10.1128/MMBR.00018-13. PMID:24006471
Original Publications
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Molecular microbiology. 2011 Sep; 81(6):1459-73. doi:10.1111/j.1365-2958.2011.07777.x. PMID:21815947
Hirooka K, Kunikane S, Matsuoka H, Yoshida K, Kumamoto K, Tojo S, Fujita Y Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids. Journal of bacteriology. 2007 Jul; 189(14):5170-82. . PMID:17483215
Seetharaman J, Kumaran D, Bonanno JB, Burley SK, Swaminathan S Crystal structure of a putative HTH-type transcriptional regulator yxaF from Bacillus subtilis. Proteins. 2006 Jun 01; 63(4):1087-91. . PMID:16475182
Yoshida K, Ohki YH, Murata M, Kinehara M, Matsuoka H, Satomura T, Ohki R, Kumano M, Yamane K, Fujita Y Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH. Journal of bacteriology. 2004 Sep; 186(17):5640-8. . PMID:15317768
Kumano M, Fujita M, Nakamura K, Murata M, Ohki R, Yamane K Lincomycin resistance mutations in two regions immediately downstream of the -10 region of lmr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants. Antimicrobial agents and chemotherapy. 2003 Jan; 47(1):432-5. . PMID:12499232

52D560AA02F0849CB24460A496021560063B2E12

Page visits: 6133

Time of last update: 2025-04-07 05:00:45

Author of last update: Jstuelk