Difference between revisions of "SigL"

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(Biological materials)
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigL_3512498_3513808_-1 sigL] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigL_3512498_3513808_-1 sigL] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:''' repressed by glucose ([[CcpA]])  {{PubMed|16166551}}
 
* '''Regulation:''' repressed by glucose ([[CcpA]])  {{PubMed|16166551}}
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* '''Mutant:'''
 
* '''Mutant:'''
** GP146 (spc), available in [[Jörg Stülke]] lab
+
** GP146 ( ''sigL''::'spc), available in [[Jörg Stülke]]'s lab {{PubMed|17183217}}
 
** 1A914 ( ''sigL''::''kan''), {{PubMed|16585774}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]
 
** 1A914 ( ''sigL''::''kan''), {{PubMed|16585774}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]
  
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==Reviews==
 
==Reviews==
 
<pubmed>7934866 10894718 21054445</pubmed>
 
<pubmed>7934866 10894718 21054445</pubmed>
 
 
==Original publications==
 
==Original publications==
 
<pubmed>16585774,11274109,1924373 ,16166551 22900538</pubmed>
 
<pubmed>16585774,11274109,1924373 ,16166551 22900538</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:31, 23 July 2014

Gene name sigL
Synonyms
Essential no
Product RNA polymerase sigma-54 factor (sigma-L)
Function utilization of arginin, acetoin and fructose,
required for cold adaptation
Gene expression levels in SubtiExpress: sigL
Interactions involving this protein in SubtInteract: SigL
MW, pI 49,5 kDa, 7.79
Gene length, protein length 1308 bp, 436 amino acids
Immediate neighbours yvfH, yvfG
Sequences Protein DNA DNA_with_flanks
Genetic context
SigL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigL expression.png
















Categories containing this gene/protein

transcription, sigma factors and their control

This gene is a member of the following regulons

CcpA regulon

The SigL regulon

The gene

Basic information

  • Locus tag: BSU34200

Phenotypes of a mutant

The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
  • Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
  • Paralogous protein(s):

Transcription factors activating transcription at SigL-dependent promoters

AcoR, BkdR, LevR, RocR, YplP

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA binding domain (H-T-H motif) (324–343)
    • pron box domain (413–421)
    • 3 x Compositional bias domain (6–21),(32–53),(112–136)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.

Transcription factors activating transcription at SigL-dependent promoters

Expression and regulation

  • Operon: sigL (according to DBTBS)
  • Regulatory mechanism: CcpA: transcription repression, transcriptional roadblock PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References

Reviews

Victoria Shingler
Signal sensory systems that impact σ⁵⁴ -dependent transcription.
FEMS Microbiol Rev: 2011, 35(3);425-40
[PubMed:21054445] [WorldCat.org] [DOI] (I p)

M Buck, M T Gallegos, D J Studholme, Y Guo, J D Gralla
The bacterial enhancer-dependent sigma(54) (sigma(N)) transcription factor.
J Bacteriol: 2000, 182(15);4129-36
[PubMed:10894718] [WorldCat.org] [DOI] (P p)

M J Merrick
In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
Mol Microbiol: 1993, 10(5);903-9
[PubMed:7934866] [WorldCat.org] [DOI] (P p)

Original publications