lutP

lutP
168

lactate permease

Locus
BSU_34190
Molecular weight
59.59 kDa
Isoelectric point
9.48
Protein length
563 aaSequenceBlast
Gene length
1692 bpSequenceBlast
Function
lactate uptake
Product
lactate permease
Essential
no
Synonyms
lutP, yvfH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1620 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,510,780 → 3,512,471
Phenotypes of a mutant
poor growth with lactate as single carbon source PubMed
The protein
Catalyzed reaction/ biological activity
lactate uptake PubMed
Protein family
lactate permease family (with LctP, according to UniProt)
Structure
P71067 (AlphaFold)
Paralogous protein(s)
LctP
Localization
membrane PubMed
Expression and Regulation
Operons
Genes
lutP
Description
PubMed
Regulation
expressed at high cell density (ComA) PubMed
Regulatory mechanism
ComA: activation, (ComA) PubMed, in comA regulon
LutR: repression, in lutR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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lutP

2025-04-04 08:07:20

ghost

84

4d0a139f9c188bf8fb1d1ab5397a4e19d54a602b

A3F48CABDA805CB9E58EED1FC0BD3CFC9B204033

Biological materials
Mutant
MGNA-A489 (yvfH::erm), available at the NBRP B. subtilis, Japan
BKE34190 (ΔlutP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCAAACCCCTCCTTGA,  downstream forward: _UP4_TAAAAAAATCCTCCCGTCTA
BKK34190 (ΔlutP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCAAACCCCTCCTTGA,  downstream forward: _UP4_TAAAAAAATCCTCCCGTCTA
GP4219 (lutP::phleo), available in Jörg Stülke's lab
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
References
Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic acids research. 2016 Mar 18; 44(5):2160-72. doi:10.1093/nar/gkv1242. PMID:26582911
Chiu KC, Lin CJ, Shaw GC Transcriptional regulation of the l-lactate permease gene lutP by the LutR repressor of Bacillus subtilis RO-NN-1. Microbiology (Reading, England). 2014 Oct; 160(Pt 10):2178-89. doi:10.1099/mic.0.079806-0. PMID:25031425
Chai Y, Kolter R, Losick R A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation. Journal of bacteriology. 2009 Apr; 191(8):2423-30. doi:10.1128/JB.01464-08. PMID:19201793
Hahne H, Wolff S, Hecker M, Becher D From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics. 2008 Oct; 8(19):4123-36. doi:10.1002/pmic.200800258. PMID:18763711
Comella N, Grossman AD Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis. Molecular microbiology. 2005 Aug; 57(4):1159-74. . PMID:16091051

E607B0D57398BCCF64CF3CE2110E632B0C3A109E

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Time of last update: 2025-04-10 22:59:46

Author of last update: Christoph.elfmann