thiO

thiO
168

FAD-dependent glycine oxidase

Locus
BSU_11670
Molecular weight
40.78 kDa
Isoelectric point
5.9
Protein length
369 aaSequenceBlast
Gene length
1110 bpSequenceBlast
Function
biosynthesis of thiamine
Product
glycine oxidase
Essential
no
E.C.
1.4.3.19
Synonyms
thiO, goxB, yjbR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0665 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,243,735  1,244,844
The protein
Catalyzed reaction/ biological activity
glycine + H2O + O2 --> glyoxylate + H2O2 + NH4+ (according to UniProt)
H2O + N-ethylglycine + O2 --> ethylamine + glyoxylate + H2O2 (according to UniProt)
H2O + O2 + sarcosine --> glyoxylate + H2O2 + methylamine (according to UniProt)
D-alanine + H2O + O2 --> H2O2 + NH4+ + pyruvate (according to UniProt)
glyphosate + H2O + O2 --> aminomethylphosphonate + glyoxylate + H+ + H2O2 (according to UniProt)
Protein family
DAO family (single member, according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
1NG3 (PDB) (complex with acetyl-glycine),  1RYI (PDB)
O31616 (AlphaFold)
Modification
FAD PubMed
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
tenA-tenI-thiO-thiS-thiG-thiF-thiD
Description
PubMed
Regulation
repressed by thiamine (Thi-box) PubMed
antitermination is modulated by NusG-dependent transcription pausing just upstream of the Thi-box PubMed
the Thi-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
Thi-box: RNA switch, via RNA switch, in Thi-box regulon
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tenA→thiD

2025-04-03 13:11:27

Jstuelk

127

A48634F4963321F6F5F59C5107F472325227A1B0

A1FEA45B91FD65F137EB4D1A0FCD88BC91331EAF

Biological materials
Mutant
MGNA-B164 (yjbR::erm), available at the NBRP B. subtilis, Japan
BKE11670 (thiO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTCCGCCTCCAATCACCA,  downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT
BKK11670 (thiO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTCCGCCTCCAATCACCA,  downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT
References
Reviews
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Original Publications
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87B5C84B7EE10F60B545CCFEF6A527A546B80432

Page visits: 2983

Time of last update: 2025-04-05 15:21:12

Author of last update: Jstuelk