yesX

yesX
168

rhamnogalacturonanlyase, degrades oligo- to disaccharides

Locus
BSU_07060
Molecular weight
67.51 kDa
Isoelectric point
5.22
Protein length
Gene length
Function
utilization of rhamnogalacturonan
Product
rhamnogalacturonan lyase, degrades oligo- to disaccharides
Essential
no
E.C.
null
Synonyms
yesX

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
772,142  773,980
The protein
Catalyzed reaction/ biological activity
Exotype eliminative cleavage of alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I oligosaccharides containing alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The products are the disaccharide 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose and the shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end (according to UniProt)
Protein family
polysaccharide lyase 11 family (with YesW, according to UniProt)
Structure
Paralogous protein(s)
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
Description
Regulation
induced by pectin PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
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yesWyesZ

2025-03-16 15:01:59

Jstuelk

96

26D24723052D02337DC2411B92E512179DD8D85B

26D24723052D02337DC2411B92E512179DD8D85B

Description
Regulation
induced by pectin (RhgR, RhgL) PubMed
repressed by glucose (CcpA) PubMed
Regulatory mechanism
RhgR: activation, PubMed, in rhgR regulon
SigA: Sigma factor, PubMed, in sigA regulon
RhgL: activation, PubMed, in rhgL regulon
CcpA: repression, PubMed, in ccpA regulon
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rhiLyesZ

2025-03-16 17:43:25

Jstuelk

117

D891C8F38E150B653BA655BA6352352D86D18BAE

A10D4124F5AF6831808648D33C98AF061D3703AA

Biological materials
Mutant
MGNA-B261 (yesX::erm), available at the NBRP B. subtilis, Japan
BKE07060 (yesX::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTTGATGGGAACGGGCTG,  downstream forward: _UP4_TAGGAGGACTAAGGGATAAG
BKK07060 (yesX::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTTGATGGGAACGGGCTG,  downstream forward: _UP4_TAGGAGGACTAAGGGATAAG
References
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217BAA94FB926CDA7CA8800BC6B08371792CEE31

Page visits: 5048

Time of last update: 2025-04-06 18:46:14

Author of last update: Melvin.boenninger