pdhD

pdhD
168

dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes

Locus
BSU_14610
Molecular weight
49.57 kDa
Isoelectric point
4.76
Protein length
470 aaSequenceBlast
Gene length
1413 bpSequenceBlast
Function
links glycolysis and TCA cycle, enzyme in TCA cycle
Product
dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes
Essential
no
E.C.
1.8.1.4
Synonyms
pdhD, citL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1249 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,531,870 1,533,282
Phenotypes of a mutant
defects in sporulation and unable to grow on glucose as single carbon source PubMed
The protein
Catalyzed reaction/ biological activity
Protein N6-(dihydrolipoyl)lysine + NAD+ --> protein N6-(lipoyl)lysine + NADH2 (according to UniProt)
Protein family
class-I pyridine nucleotide-disulfide oxidoreductase family (with AcoL and LpdV, according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
1EBD (PDB) (complex with binding domain of dihydrolipoamide acetylase, Geobacillus stearothermophilus), 1EBD (PDB) (complex with binding domain of dihydrolipoamide acetylase, Geobacillus stearothermophilus)
P21880 (AlphaFold)
Modification
phosphorylated (Ser/Thr/Tyr) PubMed
Effectors of protein activity
Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
Low sensibility to NADPH PubMed
Kinetic information
Michaelis-Menten PubMed
Paralogous protein(s)
LpdV, AcoL
Localization
cytoplasm (according to UniProt)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
pdhA-pdhB-pdhC-pdhD
Description
PubMed
Regulation
''pdhA'': expression activated by glucose (1.9-fold) PubMed
Regulatory mechanism
stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

pdhA→pdhD

2025-03-29 04:16:44

ghost

117

797fea7dcbe4317f63eb94d44165272d12ff3ae4

3D5EA61F84C1F6538A394D4F9A00FD4029ED115F

Genes
pdhC-pdhD
Description
PubMed
Regulation
''pdhA'': expression activated by glucose (1.9-fold) PubMed
Open in new tab

pdhC→pdhD

2025-04-04 07:59:38

ghost

108

22b57c718f31ad11fe44c3a9b66c0348a0c6dcab

F1C43D78FC4D596937F2D6B16CB47827D858EE6D

Biological materials
Mutant
BKE14610 (pdhD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGGAAATCTCCTACTACCA, downstream forward: _UP4_TAATTTTCATATCAAAAACA
BKK14610 (pdhD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGGAAATCTCCTACTACCA, downstream forward: _UP4_TAATTTTCATATCAAAAACA
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab
FLAG-tag construct
GP1427 (spc, based on pGP1331), available in the Jrg Stlke's lab
LacZ fusion
pGP723 (in pAC5), available in Jrg Stlke's lab
References
Reviews
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Original Publications
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E9BBAE86DF3E536A987179CC394B472F6F710498

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Time of last update: 2025-04-06 08:58:18

Author of last update: Jstuelk