pdhB

pdhB
168

pyruvate dehydrogenase (E1 beta subunit)

Locus
BSU_14590
Molecular weight
35.32 kDa
Isoelectric point
4.55
Protein length
325 aaSequenceBlast
Gene length
978 bpSequenceBlast
Function
links glycolysis and TCA cycle
Product
pyruvate dehydrogenase (E1 beta subunit)
Essential
no
E.C.
1.2.4.1
Synonyms
pdhB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0022 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,529,445 1,530,422
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
defects in sporulation and unable to grow on glucose as single carbon source PubMed
poor growth PubMed
non-transformable PubMed
The protein
Catalyzed reaction/ biological activity
[dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-lipoyl-L-lysine + H+ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-(S8-acetyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
Structure
1W88 (PDB) (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus) PubMed
P21882 (AlphaFold)
Modification
phosphorylation on (Ser-302 OR Ser-306) PubMed
Effectors of protein activity
Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
Low sensibility to NADPH
inhibited upon interaction with PdhI PubMed
Kinetic information
Michaelis-Menten PubMed
Paralogous protein(s)
AcoB, BkdAB
Localization
membrane associated PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
pdhA-pdhB-pdhC-pdhD
Description
PubMed
Regulation
''pdhA'': expression activated by glucose (1.9-fold) PubMed
Regulatory mechanism
stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pdhA→pdhD

2025-03-29 04:16:44

ghost

117

797fea7dcbe4317f63eb94d44165272d12ff3ae4

3D5EA61F84C1F6538A394D4F9A00FD4029ED115F

Biological materials
Mutant
GP459 (spc), available in Jörg Stülke's lab
BKE14590 (pdhB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA
BKK14590 (pdhB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA
Expression vectors
pGP2823 (for the purification of PdhA-PdhB-His6 in E. coli, in pET28a), available in Jörg Stülke's lab
pGP2900 (for the purification of Strep-PdhA-PdhB in E. coli, in pGP172), available in Jörg Stülke's lab
pGP3713 (for the purification of Strep-PdhA-PdhB-PdhC-PdhD in B. subtilis, in pGP380), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
LacZ fusion
pGP722 (in pAC5), available in Jörg Stülke's lab
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
References
Reviews
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Original Publications
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458E967052D1093A0F48AE0E6B6CCA0F52EAC44D

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Time of last update: 2025-04-10 04:42:22

Author of last update: Jstuelk