adaB

adaB
168

O6-methylguanine-DNA methyltransferase

Locus
BSU_01820
Molecular weight
19.98 kDa
Isoelectric point
6.15
Protein length
179 aaSequenceBlast
Gene length
540 bpSequenceBlast
Function
adaptive response to alkylative DNA damage
Product
O6-methylguanine-DNA methyltransferase
Essential
no
E.C.
2.1.1.63
Synonyms
adaB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0350 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
204,351 → 204,890
The protein
Catalyzed reaction/ biological activity
6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] --> 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] --> thymidine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
Protein family
MGMT family (with Ogt, according to UniProt)
Structure
4BHB (PDB) (from Mycobacterium tuberculosis, 42% identity) PubMed
P19220 (AlphaFold)
Expression and Regulation
Operons
Genes
adaA-adaB
Description
PubMed
Regulation
positive control by AdaA PubMed
Regulatory mechanism
AdaA: activation, in adaA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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adaA→adaB

2025-03-16 12:29:53

ghost

134

216a299d21884eb43084923c354bd4664445d33d

BD6B9F925AA4A09B6EDF781E92DBC11359A0B270

Biological materials
Mutant
BKE01820 (ΔadaB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG,  downstream forward: _UP4_TGAAAGTGAATCATCTATAA
BKK01820 (ΔadaB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG,  downstream forward: _UP4_TGAAAGTGAATCATCTATAA
References
Reviews
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Original Publications
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D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1

Page visits: 2513

Time of last update: 2025-04-04 12:12:38

Author of last update: Melvin.boenninger