argF

argF
168

ornithine carbamoyltransferase

Locus
BSU_11250
Molecular weight
34.51 kDa
Isoelectric point
4.97
Protein length
319 aaSequenceBlast
Gene length
960 bpSequenceBlast
Function
biosynthesis of arginine
Product
ornithine carbamoyltransferase
Essential
no
E.C.
2.1.3.3
Synonyms
argF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0078 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,203,461  1,204,420
The protein
Catalyzed reaction/ biological activity
carbamoyl-P + L-ornithine --> H+ + L-citrulline + phosphate (according to UniProt)
Protein family
ATCase/OTCase family (with PyrB, according to UniProt)
Structure
1A1S (PDB) (from Pyrococcus furious, 60.1% identity) PubMed
2EF0 (PDB) (from Thermus thermophilus hb8, 48% identity, 63% similarity)
P18186 (AlphaFold)
Effectors of protein activity
inhibited at high ornithine concentrations, inhibition is enhanced by interaction with RocF in the presence of exogenous arginine (1 mM) PubMed
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
argC-argJ-argB-argD-carA-carB-argF
Description
PubMed
Regulation
repressed by arginine (AhrC) PubMed
induced in the absence of RnpM PubMed
Regulatory mechanism
AhrC: repression, PubMed, in ahrC regulon
CodY: repression, PubMed, in codY regulon
RnpM: unknown, PubMed, in rnpM regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

argC→argF

2025-04-05 13:03:35

Jstuelk

123

bd8576e7d6b5ef98ec61ff0587a9708cd8d39155

DA268D208C03E6C5DAF0361E4714668AC025F3EA

Biological materials
Mutant
1A605 ( argF::erm), PubMed, available at BGSC
1A606 ( argF::erm), PubMed, available at BGSC
BKE11250 (argF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTCCGTATAAACTGGTTT,  downstream forward: _UP4_TGAGCCAAACTCAGCAGTTT
BKK11250 (argF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTCCGTATAAACTGGTTT,  downstream forward: _UP4_TGAGCCAAACTCAGCAGTTT
GP3710 (argF::aphA3 trpC2) available in the Stülke lab
Expression vectors
pGP3852: expression of argF (gapA RBS) by pBQ200 in B. subtilis, available in Jörg Stülke's lab
pGP3865: expression of argF-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
FLAG-tag construct
GP3959 argF-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
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Time of last update: 2025-04-04 12:20:58

Author of last update: Jstuelk