tpi

tpi
168

triose phosphate isomerase, glycolytic/ gluconeogenic enzyme

Locus
BSU_33920
Molecular weight
26.88 kDa
Isoelectric point
4.79
Protein length
253 aaSequenceBlast
Gene length
762 bpSequenceBlast
Function
enzyme in glycolysis/ gluconeogenesis
Product
triosephosphate isomerase
Essential
no
E.C.
5.3.1.1
Synonyms
tpi, tpiA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0149 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,479,405  3,480,166
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
essential PubMed, non-essential according to PubMed
poor growth PubMed
poorly transformable PubMed
The protein
Catalyzed reaction/ biological activity
D-glyceraldehyde-3-P --> dihydroxyacetone-P (according to UniProt)
Protein family
triosephosphate isomerase family (single member,according to UniProt)
Structure
1BTM (PDB) (complex with 2-phosphoglycolate, Geobacillus stearothermophilus)NCBI
P27876 (AlphaFold)
Modification
phosphorylation on Ser-213 PubMed
Effectors of protein activity
inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed
Localization
cytoplasm (homogeneously distributed throughout the cell) PubMed
Additional information
extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
cggR-gapA-pgk-tpi-pgm-eno
Description
PubMed
Regulation
expression induced by glycolytic intermediates (CggR) CggR PubMed
the mRNA is processed between cggR and gapA by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
CggR: repression, PubMed, in cggR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

cggR→eno

2025-03-27 06:06:35

Jstuelk

174

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

Genes
pgk-tpi-pgm-eno
Description
PubMed
Open in new tab

pgk→eno

2025-03-29 18:10:08

Jstuelk

129

c718c56bfee7b3945ef7060e9c50614ecf6b1cd7

D821B77CDDBA7B2EDE01B5B3E2E0A964498A261D

Biological materials
Mutant
GP700 (cat), available in Jörg Stülke's lab, PubMed
BKK33920 (tpi::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTTCCACTTCCTTAT,  downstream forward: _UP4_GTTCAATTATTGGAGGAAGG
Expression vectors
pGP394 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
pGP89 (N-terminal Strep-tag, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab, PubMed
pGP1511 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
References
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Time of last update: 2025-04-01 12:08:43

Author of last update: Jstuelk