pgk

pgk
168

phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, universally conserved protein

Locus
BSU_33930
Molecular weight
42.03 kDa
Isoelectric point
4.77
Protein length
394 aaSequenceBlast
Gene length
1185 bpSequenceBlast
Function
enzyme in glycolysis/ gluconeogenesis
Product
phosphoglycerate kinase
Essential
No
E.C.
2.7.2.3
Synonyms
pgk
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0126 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,480,197 3,481,381
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
essential PubMed, non-essential according to PubMed
suppression of ''ftsZ''(ts) mutation (reverted by addition of pyruvate) PubMed
poor growth PubMed
poorly transformable PubMed
The protein
Catalyzed reaction/ biological activity
3-phosphoglycerate + ATP --> 1,3-bisphosphoglycerate + ADP (according to UniProt)
Protein family
phosphoglycerate kinase family (single member, according to UniProt)
Domains
nucleotide binding domain (ATP) (350353)
2x substrate binding domain (2123), (5962)
Cofactors
Mg2 or Mn2 PubMed
Structure
1PHP (PDB) (from Geobacillus stearothermophilus)
P40924 (AlphaFold)
Modification
phosphorylation on Ser-183 AND Thr-299 PubMed, PubMed
Effectors of protein activity
Inhibited by Co2 , NDP and NMP PubMed
Kinetic information
Two Substrate Reversible Michaelis-Menten PubMed
Additional information
extensive information on the structure and enzymatic properties of Pgk can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
cggR-gapA-pgk-tpi-pgm-eno
Description
PubMed
Regulation
expression induced by glycolytic intermediates (CggR) CggR PubMed
the mRNA is processed between cggR and gapA by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
CggR: repression, PubMed, in cggR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

cggR→eno

2025-04-04 01:45:03

Jstuelk

175

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

Genes
pgk-tpi-pgm-eno
Description
PubMed
Open in new tab

pgk→eno

2025-04-03 13:01:53

Jstuelk

130

c718c56bfee7b3945ef7060e9c50614ecf6b1cd7

D821B77CDDBA7B2EDE01B5B3E2E0A964498A261D

Biological materials
Mutant
GP699 (pgk::cat), available in Jrg Stlke's lab, PubMedlab
GP707 (pgk::erm), available in Jrg Stlke's lab, PubMed
BKK33930 (pgk::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCTAGGAGATCCTCCT, downstream forward: _UP4_TAATCTCAAAACTGCTATAA
Expression vectors
pGP1102 (N-terminal His-tag, in pWH844), available in Jrg Stlke's lab
pGP95 (N-terminal Strep-tag, in pGP172), available in Jrg Stlke's lab
pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jrg Stlke's lab, PubMed
pGP1513 (expression in B. subtilis, in pBQ200), available in Jrg Stlke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available iin Jrg Stlke's lab, PubMed
LacZ fusion
pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Jrg Stlke's lab
References
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ECF0F2E906BF94F509817752827CA189AFBE53FE

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Time of last update: 2025-04-04 01:19:13

Author of last update: Jstuelk