bcrC

bcrC
168

undecaprenyl pyrophosphate phosphatase, produces the carrier lipid Und-P for cell wall synthesis (major player), secondary bacitracin resistance determinant

Locus
BSU_36530
Molecular weight
21.58 kDa
Isoelectric point
9.45
Protein length
193 aaSequenceBlast
Gene length
582 bpSequenceBlast
Function
cell wall synthesis, resistance to bacitracin and oxidative stress
Product
undecaprenyl pyrophosphate phosphatase
Essential
no
E.C.
3.6.1.27
Synonyms
bcrC, ywoA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0671 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,758,547  3,759,128
Phenotypes of a mutant
5-fold increased sensitivity to bacitracin PubMed
reduced growth rate PubMed
increased susceptibility to rare earth elements PubMed
a ''bcrC uppP double mutant is not viable (unless pgpB'' is artificially expressed) PubMed
poor growth of a bcrC sigM double mutant PubMed
poor growth of a bcrC tagV double mutant PubMed
inactivation suppresses the essentiality of mbl PubMed
The protein
Catalyzed reaction/ biological activity
undecaprenyl pyrophosphate - undecaprenyl phosphate
di-trans,octa-cis-undecaprenyl diphosphate + H2O --> di-trans,octa-cis-undecaprenyl phosphate + H+ + phosphate (according to UniProt)
Protein family
BcrC/YbjG family (single member, according to UniProt)
Structure
P94571 (AlphaFold)
Effectors of protein activity
inhibited by heptaprenyl pyrophosphate which accumulates in a ''ytpB'' mutant PubMed
Localization
cell membrane (according to Swiss-Prot)
Additional information
there is a second undecaprenyl pyrophosphate phosphatase in B. subtilis, UppP. However, UppP seems to play only a minor role. PubMed
Expression and Regulation
Operons
Genes
bcrC
Description
PubMed
Regulation
expression activated by glucose (3.3 fold) PubMed
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigI: sigma factor, PubMed, in sigI regulon
SigW: sigma factor, PubMed, in sigW regulon
SigX: sigma factor, PubMed, in sigX regulon
SigV: sigma factor, in sigV regulon
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bcrC

2025-04-06 09:16:55

Jstuelk

79

011562D3F9267D7D773C8FBB41620002798450E7

D57FA2D27F5785DF653A6EF5C57F504B07626F62

Biological materials
Mutant
MGNA-A214 (ywoA::erm), available at the NBRP B. subtilis, Japan
BKE36530 (bcrC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATAATCACCTTTTACAT,  downstream forward: _UP4_TAAGAAAGACAAAAGCCGGC
BKK36530 (bcrC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATAATCACCTTTTACAT,  downstream forward: _UP4_TAAGAAAGACAAAAGCCGGC
References
Reviews
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Original Publications
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A58729F80A0BB6683648E472E385711A6DE4D373

Page visits: 5635

Time of last update: 2025-04-10 17:29:54

Author of last update: Jstuelk