rsiX

rsiX
168

anti-SigX

Locus
BSU_23090
Molecular weight
41.15 kDa
Isoelectric point
5.45
Protein length
368 aaSequenceBlast
Gene length
1107 bpSequenceBlast
Function
control of SigX activity
Product
anti-SigX
Essential
no
E.C.
null
Synonyms
rsiX, ypuN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
2,413,585  2,414,691
Phenotypes of a mutant
inactivation of ''rsiX strongly restores beta-lactam resistance in a sigM'' mutant PubMed
increased expression of the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon PubMed
defective in Swarming motility (prolonged period of immotility prior to Swarming) PubMed
Additional information
the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots PubMed
The protein
Structure
P35166 (AlphaFold)
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
sigX-rsiX
Description
PubMed
Regulation
induced by glucose PubMed, this depends on RnpM PubMed
Regulatory mechanism
YvrHb: activation, PubMed, in yvrHb regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigX: sigma factor, PubMed, in sigX regulon
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sigX→rsiX

2025-04-11 11:32:47

Jstuelk

118

dfd4194b5990a1043dfc2b94c54efc3adc6fd3a9

F164A2C76E6C51307B22A33B48976CF41577CBAC

Biological materials
Mutant
MGNA-A180 (ypuN::erm), available at the NBRP B. subtilis, Japan
BKE23090 (rsiX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATATCCTGAGGCGAACGAT,  downstream forward: _UP4_TAAAAAGAGCCCTTTAAGGC
BKK23090 (rsiX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATATCCTGAGGCGAACGAT,  downstream forward: _UP4_TAAAAAGAGCCCTTTAAGGC
References
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21D260E900776EAAF4B6000A3365DCA9241EACA6

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Time of last update: 2025-04-14 08:24:08

Author of last update: Jstuelk