yugS

yugS
168

spore membrane protein, similar to magnesium exporter

Locus
BSU_31300
Molecular weight
49.33 kDa
Isoelectric point
5.13
Protein length
429 aaSequenceBlast
Gene length
1290 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
yugS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,216,163  3,217,452
The protein
Protein family
UPF0053 family(according to UniProt)
Domains
CNNM transmembrane domain (aa 1-201) (according to UniProt)
DUF21 domain (4-201)
2 CBS domains (aa 220-281, aa 284-341) (according to UniProt)
Structure
4HG0 (PDB) (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)
O05241 (AlphaFold)
Paralogous protein(s)
YhdT, MpfA, YqhB, YrkA
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
yugS
Description
PubMed
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yugS

2025-04-03 05:29:32

ghost

50

a674b1d01421f5cabb9529b17e7a37e2ae79cb9c

7B694A317424B34654A7111B4C925BAF5AC15147

Biological materials
Mutant
MGNA-A632 (yugS::erm), available at the NBRP B. subtilis, Japan
BKE31300 (yugS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAAAAGCCCTTACCATA,  downstream forward: _UP4_TGATAAAAAACAGCCGGGGA
BKK31300 (yugS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAAAAGCCCTTACCATA,  downstream forward: _UP4_TGATAAAAAACAGCCGGGGA
Expression vectors
pGP2936 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3466 (214-345aa) (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3471 (214-345aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
References
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Page visits: 4690

Time of last update: 2025-04-10 06:56:45

Author of last update: LKrueger