pucH

pucH
168

allantoinase

Locus
BSU_32410
Molecular weight
48.17 kDa
Isoelectric point
5.06
Protein length
446 aaSequenceBlast
Gene length
1341 bpSequenceBlast
Function
purine utilization
Product
allantoinase
Essential
no
E.C.
3.5.2.5
Synonyms
pucH, yunH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0044 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,327,247  3,328,587
The protein
Catalyzed reaction/ biological activity
allantoin + H2O --> allantoate + H+ (according to UniProt)
Protein family
Metallo-dependent hydrolases superfamily (according to UniProt)
Structure
3HM7 (PDB) (from Bacillus halodurans, 44% identity, 65% similarity)
O32137 (AlphaFold)
Paralogous protein(s)
PyrC
Expression and Regulation
Operons
Genes
pucH
Description
PubMed
Regulation
induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
Regulatory mechanism
PucR: activation, PubMed, in pucR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

pucH

2025-04-07 16:26:35

ghost

52

42f917502c5fe57e593730d32c05ff61e1088dc8

9B3A0D65F3EB8F9F7E3DE263B8C1A3AD480BFA5D

Biological materials
Mutant
MGNA-A933 (yunH::erm), available at the NBRP B. subtilis, Japan
BKE32410 (pucH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATCCCCCCATGTC,  downstream forward: _UP4_TAAGCCCGGCGGATTTTACC
BKK32410 (pucH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATCCCCCCATGTC,  downstream forward: _UP4_TAAGCCCGGCGGATTTTACC
References
Loading

8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A

Page visits: 2496

Time of last update: 2025-04-10 17:57:30

Author of last update: Jstuelk