zwf

zwf
168

glucose 6-phosphate dehydrogenase, pentose-phosphate pathway

Locus
BSU_23850
Molecular weight
55.49 kDa
Isoelectric point
5.28
Protein length
489 aaSequenceBlast
Gene length
1470 bpSequenceBlast
Function
initiation of the pentose phosphate pathway
Product
glucose 6-phosphate dehydrogenase
Essential
no
E.C.
1.1.1.49
Synonyms
zwf, yqjJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0364 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,479,156  2,480,625
Phenotypes of a mutant
suppresses the growth defect of glmR mutants on gluconeogenic substrates PubMed
a zwf pgi double mutant is unable to grow on minimal medium in the presence of glucose (due to the accumulation of toxic glucose-1-phosphate) PubMed
a zwf pgi pgcA triple mutant is not viable PubMed
The protein
Catalyzed reaction/ biological activity
D-glucose-6-P + NADP+ --> D-glucono-1,5-lactone 6-P + H+ + NADPH (according to UniProt)
Protein family
glucose-6-phosphate dehydrogenase family (single member, according to UniProt)
Cofactors
Mg2+, Mn2+, Ca2+
Structure
1DPG (PDB) (from Leuconostoc Mesenteroides, 45% identity, 62% similarity) PubMed
P54547 (AlphaFold)
Effectors of protein activity
NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed
Additional information
The enzyme is a dimer PubMed
Expression and Regulation
Operons
Genes
zwf
Description
PubMed
Regulation
constitutive PubMed
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zwf

2025-03-31 21:03:39

ghost

145

f842042450fb058200856b94a2e940c000a775a4

789DF18FA105AA04511C5B1DCF8639FE110116B4

Biological materials
Mutant
MGNA-C392 (yqjJ::erm), available at the NBRP B. subtilis, Japan
SM-NB3 (zwf-spc), available in Anne Galinier's and Boris Görke's labs PubMed
BKE23850 (zwf::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTAAAAGTACCTCACTTT,  downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG
BKK23850 (zwf::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTAAAAGTACCTCACTTT,  downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG
Expression vectors
pGP2887: expression of Strep-zwf by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
FLAG-tag construct
GP1401 (spc, based on pGP1331), available in Jörg Stülke's lab
References
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Original Publications
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43CD2581DA421CABC66E30389640CC86E6B2D800

Page visits: 6268

Time of last update: 2025-04-01 21:22:05

Author of last update: Jstuelk