yyaF

yyaF
168

GTP-binding protein/ GTPase

Locus
BSU_40920
Molecular weight
39.91 kDa
Isoelectric point
4.66
Protein length
366 aaSequenceBlast
Gene length
1101 bpSequenceBlast
Function
unknown
Product
GTP-binding protein/ GTPase
Essential
no
Synonyms
yyaF, engD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0012 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,199,843 → 4,200,943
The protein
Catalyzed reaction/ biological activity
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
Protein family
TRAFAC class OBG-HflX-like GTPase superfamily (with Obg and YnbA, according to UniProt)
Domains
OBG-typeG domain (aa 3-259) (according to UniProt)
TGS domain (aa 281-364) (according to UniProt)
Structure
1JAL (PDB) (from Haemophilus influenzae, 58% identity) PubMed
P37518 (AlphaFold)
Paralogous protein(s)
Era, YsxC, RbgA, ThdF, Obg, YqeH, YnbA, EngA
Expression and Regulation
Operons
Genes
yyzM-yyaE-yyaF-rpsF-ssbA-rpsR
Description
PubMed
Open in new tab

yyzM→rpsR

2025-03-21 16:54:05

Jstuelk

129

9d6f6c8f552e3b902331ccb27a1c555fb109c0c2

9ABCA2D017C483FB4103C07C6AB9E7C773168C9B

Genes
yyaF-rpsF-ssbA-rpsR
Description
PubMed
Regulation
ComK: transcription activation PubMed
Regulatory mechanism
ComK: activation, in comK regulon
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

yyaF→rpsR

2025-03-24 21:04:22

ghost

152

919e120921039bf82b5f231ffb9cdc8bf1c3e019

AB203DF546C89702CE168BF3D045E792086CFD74

Biological materials
Mutant
MGNA-B869 (yyaF::erm), available at the NBRP B. subtilis, Japan
BKE40920 (ΔyyaF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT,  downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG
BKK40920 (ΔyyaF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT,  downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP836, available in Stülke lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP844, available in Stülke lab
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
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Original Publications
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C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73

Page visits: 3311

Time of last update: 2025-04-04 10:41:14

Author of last update: Melvin.boenninger